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Reviewed, UniProtKB/Swiss-Prot Q9UNA3 (A4GCT_HUMAN)

Last modified September 2, 2008. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-1,4-N-acetylglucosaminyltransferase
      Short name=Alpha4GnT
    EC=2.4.1.-
Gene names
Name: A4GNT
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane proteinProbable.

Tissue specificity

Detected in stomach and pancreas.

Domain

The conserved DXD motif is involved in enzyme activity By similarity.

Sequence similarities

Belongs to the glycosyltransferase 32 family.

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Ontologies

Keywords

   Cellular componentGolgi apparatus
Membrane
   Coding sequence diversityPolymorphism
   DomainSignal-anchor
Transmembrane
   Molecular functionGlycosyltransferase
Transferase
   PTMGlycoprotein

Gene Ontology (GO)

   Biological processcarbohydrate metabolic process Ref.1

Traceable author statement. Source: ProtInc

protein amino acid O-linked glycosylation Ref.1

Traceable author statement. Source: ProtInc

   Cellular componentintegral to membrane Ref.1

Traceable author statement. Source: ProtInc

membrane fraction Ref.1

Traceable author statement. Source: ProtInc

   Molecular functionacetylglucosaminyltransferase activity Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 340340Alpha-1,4-N-acetylglucosaminyltransferase

Regions

Topological domain1 – 44Cytoplasmic Potential
Transmembrane5 – 2521Signal-anchor for type II membrane protein Potential
Topological domain26 – 340315Lumenal Potential
Motif167 – 1693DXD motif By similarity

Amino acid modifications

Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation2511N-linked (GlcNAc...) Potential
Glycosylation2821N-linked (GlcNAc...) Potential

Natural variations

Natural variant2181A → D: dbSNP rs2246945.

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Sequences

Sequence LengthMass (Da)Tools
Q9UNA3-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 00D667A8394300AB

FASTA34039,497
        10         20         30         40         50         60 
MRKELQLSLS VTLLLVCGFL YQFTLKSSCL FCLPSFKSHQ GLEALLSHRR GIVFLETSER 

        70         80         90        100        110        120 
MEPPHLVSCS VESAAKIYPE WPVVFFMKGL TDSTPMPSNS TYPAFSFLSA IDNVFLFPLD 

       130        140        150        160        170        180 
MKRLLEDTPL FSWYNQINAS AERNWLHISS DASRLAIIWK YGGIYMDTDV ISIRPIPEEN 

       190        200        210        220        230        240 
FLAAQASRYS SNGIFGFLPH HPFLWECMEN FVEHYNSAIW GNQGPELMTR MLRVWCKLED 

       250        260        270        280        290        300 
FQEVSDLRCL NISFLHPQRF YPISYREWRR YYEVWDTEPS FNVSYALHLW NHMNQEGRAV 

       310        320        330        340 
IRGSNTLVEN LYRKHCPRTY RDLIKGPEGS VTGELGPGNK

« Hide

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References

« Hide 'large scale' references
[1]"Expression cloning of a human alpha1, 4-N-acetylglucosaminyltransferase that forms GlcNAcalpha1-->4Galbeta-->R, a glycan specifically expressed in the gastric gland mucous cell-type mucin."
Nakayama J., Yeh J.-C., Misra A.K., Ito S., Katsuyama T., Fukuda M.
Proc. Natl. Acad. Sci. U.S.A. 96:8991-8996(1999) [PubMed: 10430883] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Stomach.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-218.
+Additional computationally mapped references.

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Web resources

GGDB

GlycoGene database

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Cross-references

Sequence databases

AF141315 mRNA. Translation: AAD48406.1.
BC119639 mRNA. Translation: AAI19640.1.
BC119640 mRNA. Translation: AAI19641.1.
RefSeqNP_057245.1.
UniGeneHs.278960

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblENSG00000118017. Homo sapiens. [Contig view]
GeneID51146.
KEGGhsa:51146.

Organism-specific databases

H-InvDBHIX0030726.
HGNCHGNC:17968. A4GNT.
HPAHPA008017.
PharmGKBPA134960042.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMQ9UNA3.
HOVERGENQ9UNA3.

Gene expression databases

ArrayExpressQ9UNA3.
CleanExHS_A4GNT.
GermOnlineENSG00000118017. Homo sapiens.

Family and domain databases

InterProIPR007652. Gb3_synth.
IPR007577. Glyco_trans_s_bd.
[Graphical view]
PfamPF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
[Graphical view]
ProDomQ9UNA3.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

LinkHubQ9UNA3.
ProtoNetSearch...

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Entry information

Entry nameA4GCT_HUMAN
AccessionPrimary (citable) accession number: Q9UNA3
Secondary accession number(s): Q0VDK1, Q0VDK2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: May 1, 2000
Last modified: September 2, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents