Auxin response factor 2 - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q94JM3 (ARFB_ARATH)

Last modified September 23, 2008. Version 49. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Auxin response factor 2
Alternative name(s):
    ARF1-binding protein
      Short name=ARF1-BP

Gene names

Name:	ARF2
Ordered Locus Names:	At5g62000
ORF Names:	MTG10.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression.
Subunit structure	Homo and heterodimers. Interacts with ARF1.
Subcellular location	Nucleus.
Tissue specificity	Expressed in the whole plant.
Domain	Interactions between auxin response factors (ARFs) and Aux/IAA proteins occur through their C-terminal dimerization domains III and IV.
Sequence similarities	Belongs to the ARF family. Contains 1 Aux/IAA-ARF domain. Contains 1 TF-B3 DNA-binding domain.
Caution	Was originally (Ref.4) erroneously termed IAA26 and IAA30.
Sequence caution	The sequence AAK55665.1 differs from that shown. Reason: Frameshift at position 657. The sequence AAN46837.1 differs from that shown. Reason: Frameshift at position 657.

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Ontologies

Keywords
Biological process	Auxin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
ASK7	Q39011	3	EBI-1799262,EBI-1798250

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 859

859

Auxin response factor 2

Regions

Domain

734 – 816

Aux/IAA-ARF

DNA binding

164 – 266

103

TF-B3

Experimental info

Sequence conflict

491 – 492

RR → KK Ref.3 Ref.4

Sequence conflict

520

D → E Ref.3 Ref.4

Sequence conflict

675

R → K Ref.3 Ref.4

Sequence conflict

823

P → Q Ref.3 Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

Q94JM3-1 [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: 6B75171D7FF0E66B
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FASTA

859

95,701

        10         20         30         40         50         60 
MASSEVSMKG NRGGDNFSSS GFSDPKETRN VSVAGEGQKS NSTRSAAAER ALDPEAALYR 

        70         80         90        100        110        120 
ELWHACAGPL VTVPRQDDRV FYFPQGHIEQ VEASTNQAAE QQMPLYDLPS KLLCRVINVD 

       130        140        150        160        170        180 
LKAEADTDEV YAQITLLPEA NQDENAIEKE APLPPPPRFQ VHSFCKTLTA SDTSTHGGFS 

       190        200        210        220        230        240 
VLRRHADECL PPLDMSRQPP TQELVAKDLH ANEWRFRHIF RGQPRRHLLQ SGWSVFVSSK 

       250        260        270        280        290        300 
RLVAGDAFIF LRGENGELRV GVRRAMRQQG NVPSSVISSH SMHLGVLATA WHAISTGTMF 

       310        320        330        340        350        360 
TVYYKPRTSP SEFIVPFDQY MESVKNNYSI GMRFKMRFEG EEAPEQRFTG TIVGIEESDP 

       370        380        390        400        410        420 
TRWPKSKWRS LKVRWDETSS IPRPDRVSPW KVEPALAPPA LSPVPMPRPK RPRSNIAPSS 

       430        440        450        460        470        480 
PDSSMLTREG TTKANMDPLP ASGLSRVLQG QEYSTLRTKH TESVECDAPE NSVVWQSSAD 

       490        500        510        520        530        540 
DDKVDVVSGS RRYGSENWMS SARHEPTYTD LLSGFGTNID PSHGQRIPFY DHSSSPSMPA 

       550        560        570        580        590        600 
KRILSDSEGK FDYLANQWQM IHSGLSLKLH ESPKVPAATD ASLQGRCNVK YSEYPVLNGL 

       610        620        630        640        650        660 
STENAGGNWP IRPRALNYYE EVVNAQAQAQ AREQVTKQPF TIQEETAKSR EGNCRLFGIP 

       670        680        690        700        710        720 
LTNNMNGTDS TMSQRNNLND AAGLTQIASP KVQDLSDQSK GSKSTNDHRE QGRPFQTNNP 

       730        740        750        760        770        780 
HPKDAQTKTN SSRSCTKVHK QGIALGRSVD LSKFQNYEEL VAELDRLFEF NGELMAPKKD 

       790        800        810        820        830        840 
WLIVYTDEEN DMMLVGDDPW QEFCCMVRKI FIYTKEEVRK MNPGTLSCRS EEEAVVGEGS 

       850 
DAKDAKSASN PSLSSAGNS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"ARF1, a transcription factor that binds to auxin response elements." Ulmasov T., Hagen G., Guilfoyle T.J. Science 276:1865-1868(1997) [PubMed: 9188533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 394-859. Strain: cv. Columbia.
[4]	Sessa G., Carabelli M., Ciarbelli A.R., Ruzza V., Steindler C., Ruberti I. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 406-859. Strain: cv. Columbia.
[5]	"Dimerization and DNA binding of auxin response factors." Ulmasov T., Hagen G., Guilfoyle T.J. Plant J. 19:309-319(1999) [PubMed: 10476078] [Abstract] Cited for: DIMERIZATION, TISSUE SPECIFICITY.
[6]	"Auxin-responsive gene expression: genes, promoters and regulatory factors." Hagen G., Guilfoyle T.J. Plant Mol. Biol. 49:373-385(2002) [PubMed: 12036261] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.

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Cross-references

Sequence databases
	AB016880 Genomic DNA. Translation: BAB10162.1. AF336918 mRNA. Translation: AAG53999.1. AF378862 mRNA. Translation: AAK55665.1. Frameshift. BT000784 mRNA. Translation: AAN31923.1. BT001072 mRNA. Translation: AAN46837.1. Frameshift. U89771 mRNA. Translation: AAC49752.1. Different initiation. AJ441313 mRNA. Translation: CAD29696.1. Different initiation. AJ458328 mRNA. Translation: CAD30210.1. Different initiation.
RefSeq	NP_201006.2. NP_851244.1. NP_974980.1.
UniGene	At.21704
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q94JM3.
Genome annotation databases
GeneID	836321.
GenomeReviews	Gene locus AT5G62000 in contig BA000015_GR.
KEGG	ath:AT5G62000.
NMPDR	fig\|3702.1.peg.28232.
Organism-specific databases
GeneFarm	3364. 345.
TAIR	At5g62000.
Gene expression databases
ArrayExpress	Q94JM3.
GermOnline	AT5G62000. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003311. AUX_IAA. IPR011525. AuxIAA_ARF_dimer. IPR010525. Auxin_resp. IPR003340. TF_B3. [Graphical view]
Pfam	PF02309. AUX_IAA. 1 hit. PF06507. Auxin_resp. 1 hit. PF02362. B3. 1 hit. [Graphical view]
PROSITE	PS50863. B3. 1 hit. PS50962. IAA_ARF. 1 hit. [Graphical view]
ProDom	Q94JM3. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

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Entry information

Entry name

ARFB_ARATH

Accession

Primary (citable) accession number: Q94JM3
Secondary accession number(s): O23671, Q9C5W5, Q9FIT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	September 23, 2008
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents