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Reviewed, UniProtKB/Swiss-Prot Q8L7G0 (ARFA_ARATH)

Last modified July 22, 2008. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Auxin response factor 1
Gene names
Name: ARF1
Ordered Locus Names: At1g59750
ORF Names: F23H11.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length665 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression.

Subunit structure

Homo and heterodimers. Interacts with the auxin-responsive proteins IAA12, IAA13, IAA17 and with ARF2.

Subcellular location

Nucleus.

Tissue specificity

Expressed in the whole plant.

Domain

Interactions between auxin response factors (ARFs) and Aux/IAA proteins occur through their C-terminal dimerization domains III and IV.

Sequence similarities

Belongs to the ARF family.

Contains 1 Aux/IAA-ARF domain.

Contains 1 TF-B3 DNA-binding domain.

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Ontologies

Keywords

   Biological processAuxin signaling pathway
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Molecular functionRepressor
   Technical termComplete proteome

Gene Ontology (GO)

None. [Check GOA]

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8L7G0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8L7G0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     390-392: Missing.
Notes: May be due to a competing acceptor splice site. No experimental confirmation available.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 665665Auxin response factor 1

Regions

Domain543 – 62583Aux/IAA-ARF
DNA binding124 – 226103TF-B3
Compositional bias344 – 41269Pro-rich

Natural variations

Alternative sequence390 – 3923Missing in isoform 2.

Experimental info

Sequence conflict1361H → Q in AAM91657. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: 79DD3180C2091401

FASTA66573,668
        10         20         30         40         50         60 
MAASNHSSGK PGGVLSDALC RELWHACAGP LVTLPREGER VYYFPEGHME QLEASMHQGL 

        70         80         90        100        110        120 
EQQMPSFNLP SKILCKVINI QRRAEPETDE VYAQITLLPE LDQSEPTSPD APVQEPEKCT 

       130        140        150        160        170        180 
VHSFCKTLTA SDTSTHGGFS VLRRHADDCL PPLDMSQQPP WQELVATDLH NSEWHFRHIF 

       190        200        210        220        230        240 
RGQPRRHLLT TGWSVFVSSK KLVAGDAFIF LRGENEELRV GVRRHMRQQT NIPSSVISSH 

       250        260        270        280        290        300 
SMHIGVLATA AHAITTGTIF SVFYKPRTSR SEFIVSVNRY LEAKTQKLSV GMRFKMRFEG 

       310        320        330        340        350        360 
EEAPEKRFSG TIVGVQENKS SVWHDSEWRS LKVQWDEPSS VFRPERVSPW ELEPLVANST 

       370        380        390        400        410        420 
PSSQPQPPQR NKRPRPPGLP SPATGPSGPV TPDGVWKSPA DTPSSVPLFS PPAKAATFGH 

       430        440        450        460        470        480 
GGNKSFGVSI GSAFWPTNAD SAAESFASAF NNESTEKKQT NGNVCRLFGF ELVENVNVDE 

       490        500        510        520        530        540 
CFSAASVSGA VAVDQPVPSN EFDSGQQSEP LNINQSDIPS GSGDPEKSSL RSPQESQSRQ 

       550        560        570        580        590        600 
IRSCTKVHMQ GSAVGRAIDL TRSECYEDLF KKLEEMFDIK GELLESTKKW QVVYTDDEDD 

       610        620        630        640        650        660 
MMMVGDDPWN EFCGMVRKIF IYTPEEVKKL SPKNKLAVNA RMQLKADAEE NGNTEGRSSS 


MAGSR

« Hide

Isoform 2 [UniParc].

Checksum: 598A0403F161FA54
Show »

66273,370

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References

« Hide 'large scale' references
[1]"ARF1, a transcription factor that binds to auxin response elements."
Ulmasov T., Hagen G., Guilfoyle T.J.
Science 276:1865-1868(1997) [PubMed: 9188533] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Columbia.
[4]"Dimerization and DNA binding of auxin response factors."
Ulmasov T., Hagen G., Guilfoyle T.J.
Plant J. 19:309-319(1999) [PubMed: 10476078] [Abstract]
Cited for: DIMERIZATION, TISSUE SPECIFICITY.
[5]"Activation and repression of transcription by auxin-response factors."
Ulmasov T., Hagen G., Guilfoyle T.J.
Proc. Natl. Acad. Sci. U.S.A. 96:5844-5849(1999) [PubMed: 10318972] [Abstract]
Cited for: TRANSCRIPTIONAL REPRESSOR.
[6]"Auxin-responsive gene expression: genes, promoters and regulatory factors."
Hagen G., Guilfoyle T.J.
Plant Mol. Biol. 49:373-385(2002) [PubMed: 12036261] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.

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Cross-references

Sequence databases

U83245 mRNA. Translation: AAC49751.1.
AC007258 Genomic DNA. Translation: AAD39318.1.
AY133723 mRNA. Translation: AAM91657.1.
BT002748 mRNA. Translation: AAO22577.1.
PIRD96621.
RefSeqNP_001031208.1.
NP_176184.1.
NP_849830.1.
UniGeneAt.10812

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID842268.
GenomeReviewsGene locus AT1G59750 in contig CT485782_GR.
KEGGath:AT1G59750.
NMPDRfig|3702.1.peg.5361.

Organism-specific databases

GeneFarm3363. 345.
TAIRAt1g59750.

Gene expression databases

ArrayExpressQ8L7G0.
GermOnlineAT1G59750. Arabidopsis thaliana.

Family and domain databases

InterProIPR011525. AuxIAA_ARF_dimer.
IPR010525. Auxin_resp.
IPR003340. TF_B3.
[Graphical view]
PfamPF06507. Auxin_resp. 1 hit.
PF02362. B3. 1 hit.
[Graphical view]
PROSITEPS50863. B3. 1 hit.
PS50962. IAA_ARF. 1 hit.
[Graphical view]
ProDomQ8L7G0.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameARFA_ARATH
AccessionPrimary (citable) accession number: Q8L7G0
Secondary accession number(s): O23664
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: July 22, 2008
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents