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Reviewed, UniProtKB/Swiss-Prot Q6J5K9 (ARMI_DROME)

Last modified June 10, 2008. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesProbable RNA helicase armi
Also known as:
     EC 3.6.1.-
     Armitage protein
Gene names
Name: armi
ORF Names: CG11513
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Probable RNA helicase required for axial polarization of the oocyte during early and mid oogenesis. Plays a central role in RNA interference (RNAi) process, a process that mediates mRNA destruction of translational repression. Required for the assembly of the RISC complex, a complex required for target RNA destruction or repression. May be required in the RISC assembly to unwind miRNAs, in the production of single-stranded miRNA from the double-stranded miRNA, a key step in RISC formation. Required both for the translational control of oskar (osk) mRNA and cytoskeletal polarization in the oocyte.

Subcellular location

Cytoplasm.

Tissue specificity

Abundant in oocytes and syncitial blastoderm. Expressed at low level throughout development, including somatic tissues. First apparent early in oogenesis, in the cytoplasm of stem cells and mitotically dividing cystoblasts. In regions 2a and 2b of the germarium, it is most concentrated in the center of the germline cysts, where the pro-oocyte is located. In stage 1 and early stage 2 egg chambers, it accumulates at the anterior of the oocyte, near the ring canals. It also extends through the ring canals forming a branched structure that links the early oocyte with adjacent nurse cells. In stage 3 cysts, it accumulates at the posterior cortex and localizes to extensions that pass through the oocyte into the nurse cells. Through stages 4 to 7, it continues to be somewhat enriched at the posterior cortex of the oocyte, but at significantly lower level. In stage 9 to 10 egg chambers, it is found throughout the cytoplasm of the oocyte and nurse cells, with slight enrichment at the oocyte cortex.

Sequence similarities

Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q6J5K9-1)
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Notes: No experimental confirmation available.

Isoform B (identifier: Q6J5K9-2)
The sequence of this isoform differs from the canonical sequence as follows:
     1-86: Missing.
     87-92 DKKAAH → MFTYVS

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 12741274Probable RNA helicase armi

Regions

Nucleotide binding809 – 8168ATP Potential
Motif948 – 9514DEAG box

Natural variations

Alternative sequence1 – 8686Missing in isoform B.
Alternative sequence87 – 926DKKAAH → MFTYVS in isoform B.

Experimental info

Sequence conflict6081T → K in AAT12000. Ref.1
Sequence conflict6131I → V in AAT12000. Ref.1
Sequence conflict9911S → L in AAT12000. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified October 25, 2004. Version 3.
Checksum: C340E2B5E4F75653

FASTA1,274144,889
        10         20         30         40         50         60 
MSSEASGSIT IKEEASTSGT ATNIPVESSE TEAKKPEIGA DKAESQSETT EGGKAAETTE 

        70         80         90        100        110        120 
NHLKRLQNLR KELSYLSETD WMYESLDKKA AHKFFTNPDR NREDILESLD RENSFLDQKL 

       130        140        150        160        170        180 
MEEKMDQQLK ANPNEINGVL SNKIAELTHG LSEMDVSKES SCTARKGVIT SLDGDRGVID 

       190        200        210        220        230        240 
KDVLFETKVA EDIILDLHVG CVVEYLTFTT GEAMRVVKVK SILEHSWEDT SQKEIEKAVD 

       250        260        270        280        290        300 
NLKNEKPTFF NTETRSVLGL ISQRLASSID VETEYGQLTV ELDNIEMNFI PTNGDRVRLE 

       310        320        330        340        350        360 
CNIQLDDGFV DKQGEILEVT KLFPTRIQEG EKCIVERVYV HMVVLGPETY ILKTDLPTGT 

       370        380        390        400        410        420 
DLHLGDIVLA DLIECQYSKF TRRAIKITPL EKNFGATKLT QQSSMGSSGS GKAVTVTGVN 

       430        440        450        460        470        480 
RFITAELWQK ESVSLKLTNN LNRTLRLESI TVCNDSESQL SVVSPLESKE ISSGSEITVT 

       490        500        510        520        530        540 
FEIHTQFLGE AIEKYVLNFD LLKVRRVFTV IVCKTKEEVA EAEKRMIAAE ALMAPGRNSQ 

       550        560        570        580        590        600 
ERSRFYANQV WCNKVDVIPG QQIVTKRRFV ALRLGCFEVP KELRQICLTS ERRQEMIKAI 

       610        620        630        640        650        660 
EQHYSFLTEP LSIKTYMHRF RLLLHLEEIE CFVNFRNYDR DRAHFLRDGE FLTLQIENLA 

       670        680        690        700        710        720 
ERRPSLVIGD TLRVINPWSD PDSQTTKSYE GIIHKVLFDR ILLKFHSSFQ EKYNGEDYRL 

       730        740        750        760        770        780 
EFYFSRYSFR KQHHAISKIV GVMGEDFLFP SKVTKRENPQ LDVYMKDDDM YLYDSKLEWY 

       790        800        810        820        830        840 
NQSLNSIQKR AVFNILRGEA ENIPYVLFGP PGSGKTMTLI ETLLQLVRNL PGARILVGTP 

       850        860        870        880        890        900 
SNSSADLVTK RLIDSKALLQ GDFIRLVSYN QVEKDLIPPE IMSYCATSDV GAVGSCEDKM 

       910        920        930        940        950        960 
MVTESGLKLR CQAKFIGTHR ITISTCTTLG NFLQLGFPAG HFTHVLFDEA GQCTEPETMV 

       970        980        990       1000       1010       1020 
PIVMLTKKRS QVVLSGDPRQ LQSIVTSRIA SKMGFSISFL ERLLERSPYR KDLQRFPESS 

      1030       1040       1050       1060       1070       1080 
GYNPLVLTKL LYNYRALPSI MSIYSRLFYD DELIPVLSEK DSRESRLLSK LRCVFESEKD 

      1090       1100       1110       1120       1130       1140 
IPQAHGTFFY GIIGENRQNN DSPSWFNPQE VREVFLMTIA LYRANVTADQ IGIITPYQKQ 

      1150       1160       1170       1180       1190       1200 
VKMLRSMFIG TDVVMPKIGS VEEFQGQERD IILISTVRSS EEILRMDARF SLGFVRCSKR 

      1210       1220       1230       1240       1250       1260 
LNVAVSRARA MMIIFGNPHL LAVDECWRQL ILFCVKNNAY FGCDLPQMVI NQKDEVPVCL 

      1270 
ETFVPSLNTT DDLN

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Isoform B [UniParc].

Checksum: BA1D8F4305825B0A
Show »

1,188135,658

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References

« Hide 'large scale' references
[1]"The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification."
Cook H.A., Koppetsch B.S., Wu J., Theurkauf W.E.
Cell 116:817-829(2004) [PubMed: 15035984] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Ovary.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]"RISC assembly defects in the Drosophila RNAi mutant armitage."
Tomari Y., Du T., Haley B., Schwarz D.S., Bennett R., Cook H.A., Koppetsch B.S., Theurkauf W.E., Zamore P.D.
Cell 116:831-841(2004) [PubMed: 15035985] [Abstract]
Cited for: FUNCTION.

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Cross-references

Sequence databases

AY598469 mRNA. Translation: AAT12000.1.
AE014296 Genomic DNA. Translation: AAF47775.1.
AE014296 Genomic DNA. Translation: AAX52729.1.
RefSeqNP_001014556.1.
NP_647816.1.
UniGeneDm.15753

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblCG11513. Drosophila melanogaster. [Contig view]
GeneID38427.
KEGGdme:Dmel_CG11513.
NMPDRfig%7C7227.3.peg.7955.

Organism-specific databases

FlyBaseFBgn0041164. armi.

Phylogenomic databases

HOGENOMQ6J5K9.

Gene expression databases

ArrayExpressQ6J5K9.
GermOnlineCG11513. Drosophila melanogaster.

Family and domain databases

ProDomQ6J5K9.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameARMI_DROME
AccessionPrimary (citable) accession number: Q6J5K9
Secondary accession number(s): Q59E56, Q9VZP4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 25, 2004
Last modified: June 10, 2008
This is version 33 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents