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Reviewed, UniProtKB/Swiss-Prot Q64654 (CP51A_RAT)

Last modified September 2, 2008. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 51A1
    EC=1.14.13.70
Alternative name(s):
    CYPLI
    P450LI
    Sterol 14-alpha demethylase
    Lanosterol 14-alpha demethylase
      Short name=LDM
    P450-14DM
Gene names
Name: Cyp51a1
Synonyms: Cyp51
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Catalytic activity

Obtusifoliol + 3 O(2) + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP(+) + 4 H(2)O.

Cofactor

Heme group By similarity.

Pathway

Steroid metabolism; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.

Subcellular location

Endoplasmic reticulum membranePotential. Microsome membranePotential.

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Biological processCholesterol biosynthesis
Lipid synthesis
Steroid biosynthesis
Sterol biosynthesis
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   DomainTransmembrane
   LigandHeme
Iron
Metal-binding
NADP
   Molecular functionMonooxygenase
Oxidoreductase

Gene Ontology (GO)

   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 503503Cytochrome P450 51A1

Regions

Transmembrane24 – 4421 Potential

Sites

Metal binding4491Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict1811E → K in AAA87074. Ref.4

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Sequences

Sequence LengthMass (Da)Tools
Q64654-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 33D8F345FFE9CF21

FASTA50356,707
        10         20         30         40         50         60 
MVLLGLLQSG GSVLGQAMEQ VTGGNLLSTL LIACAFTLSL VYLFRLAVGH MVQLPAGAKS 

        70         80         90        100        110        120 
PPYIYSPIPF LGHAIAFGKS PIEFLENAYE KYGPVFSFTM VGKTFTYLLG SDAAALLFNS 

       130        140        150        160        170        180 
KNEDLNAEEV YGRLTTPVFG KGVAYDVPNA VFLEQKKILK SGLNIAHFKQ YVSIIEKEAK 

       190        200        210        220        230        240 
EYFKSWGESG ERNVFEALSE LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFSHAAWL 

       250        260        270        280        290        300 
LPGWLPLPSF RRRDRAHREI KNIFYKAIQK RRLSKEPAED ILQTLLDSTY KDGRPLTDDE 

       310        320        330        340        350        360 
IAGMLIGLLL AGQHTSSTTS AWMGFFLARD KPLQDKCYLE QKTVCGEDLP PLTYEQLKDL 

       370        380        390        400        410        420 
NLLDRCIKET LRLRPPIMTM MRMAKTPQTV AGYTIPPGHQ VCVSPTVNQR LKDSWVERLD 

       430        440        450        460        470        480 
FNPDRYLQDN PASGEKFAYV PFGAGRHRCI GENFAYVQIK TIWSTMLRLY EFDLINGYFP 

       490        500 
SVNYTTMIHT PENPVIRYKR RSK

« Hide

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References

« Hide 'large scale' references
[1]"Occurrence of a P450 showing high homology to yeast lanosterol 14-demethylase (P450(14DM)) in the rat liver."
Aoyama Y., Funae Y., Noshiro M., Horiuchi T., Yoshida Y.
Biochem. Biophys. Res. Commun. 201:1320-1326(1994) [PubMed: 8024575] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Structural and evolutionary studies on sterol 14-demethylase P450 (CYP51), the most conserved P450 monooxygenase: I. Structural analyses of the gene and multiple sizes of mRNA."
Noshiro M., Aoyama Y., Kawamoto T., Gotoh O., Horiuchi T., Yoshida Y.
J. Biochem. 122:1114-1121(1997) [PubMed: 9498553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Wistar.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[4]"Cloning and functional expression of the cDNA encoding rat lanosterol 14-alpha demethylase."
Sloane D.L., So O.Y., Leung R., Scarafia L.E., Saldou N., Jarnagin K., Swinney D.C.
Gene 161:243-248(1995) [PubMed: 7665087] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-503.
Tissue: Liver.

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Cross-references

Sequence databases

D55681 mRNA. Translation: BAA09529.1.
AB004096 Genomic DNA. Translation: BAA20354.1.
BC087033 mRNA. Translation: AAH87033.1.
U17697 mRNA. Translation: AAA87074.1.
PIRJC4240.
JC4758.
RefSeqNP_037073.1.
UniGeneRn.107152

3D structure databases

HSSPHSSP built from PDB template 1E9X based on UniProtKB P77901.
ModBaseSearch...

Genome annotation databases

EnsemblENSRNOG00000007234. Rattus norvegicus. [Contig view]
GeneID25427.
KEGGrno:25427.

Organism-specific databases

RGD2481. Cyp51.

Phylogenomic databases

HOVERGENQ64654.

Gene expression databases

ArrayExpressQ64654.
GermOnlineENSRNOG00000007234. Rattus norvegicus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00465. EP450IV.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomQ64654.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameCP51A_RAT
AccessionPrimary (citable) accession number: Q64654
Secondary accession number(s): Q64549
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 2, 2008
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents