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Reviewed, UniProtKB/Swiss-Prot Q64441 (CP24A_MOUSE)

Last modified July 22, 2008. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
      Short name=Vitamin D(3) 24-hydroxylase
      Short name=24-OHase
    EC=1.14.13.n4
Alternative name(s):
    Cytochrome P450 24A1
    P450-CC24
Gene names
Name: Cyp24a1
Synonyms: Cyp-24, Cyp24
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid.

Catalytic activity

Calcitriol + NADPH + O(2) = calcitetrol + NADP(+) + H(2)O.

Calcidiol + NADPH + O(2) = secalciferol + NADP(+) + H(2)O.

Cofactor

Heme group By similarity.

Subcellular location

Mitochondrion.

Induction

By 1,25-dihydroxyvitamin D(3) in kidney.

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Cellular componentMitochondrion
   DomainTransit peptide
   LigandHeme
Iron
Metal-binding
NADP
   Molecular functionMonooxygenase
Oxidoreductase

Gene Ontology (GO)

   Biological processvitamin D metabolic process

Inferred from mutant phenotype. Source: MGI

   Cellular componentmitochondrion

Inferred from direct assay. Source: MGI

   Molecular function25-hydroxycholecalciferol-24-hydroxylase activity

Inferred from mutant phenotype. Source: MGI

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Transit peptide1 – 3535Mitochondrion By similarity
Chain36 – 5144791,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Sites

Metal binding4621Iron (heme axial ligand) Potential

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Sequences

Sequence LengthMass (Da)Tools
Q64441-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3D38BA9235177A42

FASTA51459,453
        10         20         30         40         50         60 
MSCPIDKRRP LIAFLRRLRD LGQPPRSVTS KAHVKRAPKE VPLCPLMTDG ETRNVTSLPG 

        70         80         90        100        110        120 
PTNWPLLGSL LEIFWKGGLK KQHDTLAEYH KKYGQIFRMK LGSFDSVHLG SPSLLEALYR 

       130        140        150        160        170        180 
TESAHPQRLE IKPWKAYRDH RNEAYGLMIL EGQEWQRVRS AFQKKLMKPV EIMKLDKKIN 

       190        200        210        220        230        240 
EVLADFMGQI DELRDERGRI QDLYSELNKW SFESICLVLY EKRFGLLQKD TEEEALTFIA 

       250        260        270        280        290        300 
AIKTMMSTFG KMMVTPVELH KRLNTKVWQA HTLAWDTIFK SVKPCIDHRL ERYSQQPGAD 

       310        320        330        340        350        360 
FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQVQQ RLLREIQSVL 

       370        380        390        400        410        420 
PDNQTPRAED VRNMPYLKAC LKESMRLTPS VPFTTRTLDK PTVLGEYTLP KGTVLTLNTQ 

       430        440        450        460        470        480 
VLGSSEDNFE DADKFRPERW LEKEKKINPF AHLPFGVGKR MCIGRRLAEL QLHLALCWII 

       490        500        510 
QKYNIVATDS EPVEMLHLGI LVPSRELPIA FCPR

« Hide

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References

[1]"Molecular cloning of 25-hydroxyvitamin D-3 24-hydroxylase (Cyp-24) from mouse kidney: its inducibility by vitamin D-3."
Itoh S., Yoshimura T., Iemura O., Yamada E., Tsujikawa K., Kohama Y., Mimura T.
Biochim. Biophys. Acta 1264:26-28(1995) [PubMed: 7578252] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Kidney.
[2]"Mouse vitamin D-24-hydroxylase: molecular cloning, tissue distribution, and transcriptional regulation by 1alpha,25-dihydroxyvitamin D3."
Akeno N., Saikatsu S., Kawane T., Horiuchi N.
Endocrinology 138:2233-2240(1997) [PubMed: 9165006] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ddY.
Tissue: Kidney.
[3]"Klotho, a gene related to a syndrome resembling human premature aging, functions in a negative regulatory circuit of vitamin D endocrine system."
Tsujikawa H., Kurotaki Y., Fujimori T., Fukuda K., Nabeshima Y.
Mol. Endocrinol. 17:2393-2403(2003) [PubMed: 14528024] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

D49438 mRNA. Translation: BAA08416.1.
D89669 mRNA. Translation: BAA21843.1.
PIRS60033.
RefSeqNP_034126.1.
UniGeneMm.6575

3D structure databases

ModBaseSearch...

PTM databases

PhosphoSiteQ64441.

Genome annotation databases

EnsemblENSMUSG00000038567. Mus musculus. [Contig view]
GeneID13081.
KEGGmmu:13081.

Organism-specific databases

MGIMGI:88593. Cyp24a1.

Phylogenomic databases

HOGENOMQ64441.
HOVERGENQ64441.

Gene expression databases

ArrayExpressQ64441.
GermOnlineENSMUSG00000038567. Mus musculus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR002949. Cyt_P450_E_CYP24A_mit.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01238. MITP450CC24.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomQ64441.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

SOURCESearch...
ProtoNetSearch...

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Entry information

Entry nameCP24A_MOUSE
AccessionPrimary (citable) accession number: Q64441
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 22, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents