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Reviewed, UniProtKB/Swiss-Prot Q60996 (2A5G_MOUSE)

Last modified July 22, 2008. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
Alternative name(s):
    PP2A, B subunit, B' gamma isoform
    PP2A, B subunit, B56 gamma isoform
    PP2A, B subunit, PR61 gamma isoform
    PP2A, B subunit, R5 gamma isoform
    PP2A, B subunit, B'alpha3 isoform
Gene names
Name: Ppp2r5c
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Interacts with cyclin G in vitro. Isoform 3 may damage the checkpoint, which may then allow the acquisition of genetic alterations that promote metastasis.

Subunit structure

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Subcellular location

CytoplasmBy similarity.

Tissue specificity

Highest levels in heart, liver and brain. Lower levels in skeletal muscle, spleen, kidney and lung.

Sequence similarities

Belongs to the phosphatase 2A regulatory subunit B56 family.

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Ontologies

Keywords

   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   PTMPhosphoprotein

Gene Ontology (GO)

   Cellular componentnucleus

Traceable author statement. Source: MGI

   Molecular functionprotein binding Ref.3

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

PPP2R1AP301531EBI-1369292,EBI-302388From a different organism.

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: Q60996-1)

Also known as: Gamma-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q60996-2)

Also known as: Gamma-1;

The sequence of this isoform differs from the canonical sequence as follows:
     443-452: YAVYSQASAV → VLKKRVTREC
     453-524: Missing.
Notes: No experimental confirmation available.
Isoform 3 (identifier: Q60996-3)

Also known as: Gamma-3;

The sequence of this isoform differs from the canonical sequence as follows:
     442-480: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 524524Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform

Amino acid modifications

Modified residue3481Phosphotyrosine By similarity
Modified residue3491Phosphotyrosine By similarity
Modified residue3541Phosphotyrosine By similarity

Natural variations

Alternative sequence442 – 48039Missing in isoform 3.
Alternative sequence443 – 45210YAVYSQASAV → VLKKRVTREC in isoform 1.
Alternative sequence453 – 52472Missing in isoform 1.

Experimental info

Sequence conflict17 – 193SSN → APT in BAB32447 and BAB32448. Ref.2
Sequence conflict321D → N in AAB70857. Ref.4
Sequence conflict721A → S Ref.3 Ref.4

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Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (Gamma-2) [UniParc].

Last modified July 19, 2005. Version 2.
Checksum: A22DFD34A1FFBD01

FASTA52460,824
        10         20         30         40         50         60 
MLTCNKAGSG MVVDAASSNG PFQPVALLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL 

        70         80         90        100        110        120 
SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEAVHMFA VNMFRTLPPS SNPTGAEFDP 

       130        140        150        160        170        180 
EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD 

       190        200        210        220        230        240 
FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK 

       250        260        270        280        290        300 
EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK 

       310        320        330        340        350        360 
EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS 

       370        380        390        400        410        420 
DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK 

       430        440        450        460        470        480 
LKMKEREEAW VKIENLAKAN PQYAVYSQAS AVSIPVAMET DGPQFEDVQM LKKTVSDEAR 

       490        500        510        520 
QAQKELKKDR PLVRRKSELP QDPHTEKALE AHCRASELLS QDGR

« Hide

Isoform 1 (Gamma-1) [UniParc].

Checksum: DFD00AF40F422088
Show »

45252,818
Isoform 3 (Gamma-3) [UniParc].

Checksum: E5BE84FF6E3E01C7
Show »

48556,552

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References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
[2]"A truncated isoform of the protein phosphatase 2A B56gamma regulatory subunit may promote genetic instability and cause tumor progression."
Ito A., Koma Y., Watabe K., Nagano T., Endo Y., Nojima H., Kitamura Y.
Am. J. Pathol. 162:81-91(2003) [PubMed: 12507892] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-524 (ISOFORMS 2 AND 3).
[3]"Identification of a novel protein phosphatase 2A regulatory subunit highly expressed in muscle."
Tehrani M.A., Mumby M.C., Kamibayashi C.
J. Biol. Chem. 271:5164-5170(1996) [PubMed: 8617797] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-524 (ISOFORM 1).
Tissue: T-cell.
[4]"Identification by differential display of annexin-VI, a gene differentially expressed during melanoma progression."
Francia G., Mitchell S.D., Moss S.E., Hanby A.M., Marshall J.F., Hart I.R.
Cancer Res. 56:3855-3858(1996) [PubMed: 8752144] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-524 (ISOFORM 1).
Strain: C57BL/6.
+Additional computationally mapped references.

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Cross-references

Sequence databases

BC003979 mRNA. Translation: AAH03979.1.
AB055635 mRNA. Translation: BAB32447.1.
AB055636 mRNA. Translation: BAB32448.1.
U37353 mRNA. Translation: AAC52435.1.
U59418 mRNA. Translation: AAB70857.1.
RefSeqNP_001074926.1.
NP_036153.2.
UniGeneMm.240396

3D structure databases

SMRQ60996. Positions 30-406.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:24181N.
IntActQ60996.

PTM databases

PhosphoSiteQ60996.

Genome annotation databases

EnsemblENSMUSG00000017843. Mus musculus. [Contig view]
GeneID26931.
KEGGmmu:26931.

Organism-specific databases

MGIMGI:1349475. Ppp2r5c.

Phylogenomic databases

HOGENOMQ60996.
HOVERGENQ60996.

Gene expression databases

ArrayExpressQ60996.
GermOnlineENSMUSG00000017843. Mus musculus.

Family and domain databases

InterProIPR002554. PP2A_B56.
[Graphical view]
PANTHERPTHR10257. B56. 1 hit.
PfamPF01603. B56. 1 hit.
[Graphical view]
PIRSFPIRSF028043. PP2A_B56. 1 hit.
ProDomQ60996.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

LinkHubQ60996.
SOURCESearch...
ProtoNetSearch...

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Entry information

Entry name2A5G_MOUSE
AccessionPrimary (citable) accession number: Q60996
Secondary accession number(s): O35708 expand/collapse secondary AC list , Q99KW8, Q99N67, Q99N68
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 19, 2005
Last modified: July 22, 2008
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents