Serine/threonine-protein kinase ATM - Drosophila melanogaster (Fruit fly)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase ATM
    EC=2.7.11.1
Alternative name(s):
    Telomere fusion protein

Gene names

Name:	tefu
Synonyms:	atm
ORF Names:	CG6535

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length	2767 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Serine/threonine-protein kinase which recognizes the substrate consensus sequence [ST]-Q. Required to suppress spontaneous apoptosis of proliferating cells during development, and for their proper differentiation. Required for female fertility. Protects telomeres from fusion, maybe by recruiting or maintaining chromatin-modifying complexes such as Su(var)205/HP1. May activate checkpoint signaling in response to DNA double-stranded breaks induced by low-dose ionizing radiation. May phosphorylate histone H2AV.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	NucleusProbable. Telomere.
Developmental stage	Expressed at low levels throughout development and at much higher levels in adult females compared to males.
Miscellaneous	Flies lacking tefu die shortly before or after eclosion with a rough eye phenotype, misshapen wings, and missing or abnormal bristles.
Sequence similarities	Belongs to the PI3/PI4-kinase family. ATM subfamily. Contains 1 FAT domain. Contains 1 FATC domain. Contains 1 PI3K/PI4K domain.

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Ontologies

Keywords
Biological process	Cell cycle DNA damage DNA repair
Cellular component	Chromosomal protein Nucleus Telomere
Molecular function	Chromatin regulator Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G2/M transition DNA damage checkpoint Ref.9 Inferred from mutant phenotype. Source: FlyBase chromatin silencing at telomere Ref.8 Inferred from mutant phenotype. Source: FlyBase phosphorylation Non-traceable author statement. Source: FlyBase telomere capping Inferred from mutant phenotype. Source: FlyBase
Molecular function	protein kinase activity Non-traceable author statement. Source: FlyBase
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 2767

2767

Serine/threonine-protein kinase ATM

PRO_0000225627

Regions

Domain

1649 – 2317

669

FAT

Domain

2443 – 2696

254

PI3K/PI4K

Domain

2735 – 2767

33

FATC

Experimental info

Sequence conflict

2733

1

T → P in AAM29197. Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

Q5EAK6-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: F354E55FA1B35570
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FASTA

2,767

317,991

        10         20         30         40         50         60 
MSALLNEIQR ILGDLQSGKA ASRNKGIQQL DEKLSSCRED LDKLFLSKTS DISWTTIFEA 

        70         80         90        100        110        120 
SKEALFKQAG NLEDVNEKVF KTLAGKNYLY DNVLEKITQF NLEAGSQTSG NGHFLAKTSI 

       130        140        150        160        170        180 
FSAFEDGIKM RVVVKYFGDR ILSLLEKGIY SSVSYVRDLK INEYSRILSY LFELNVDMDE 

       190        200        210        220        230        240 
VLRTRILKCI TRTVSLAKDR VQLHVDLVEY LPELSSFALS AFGARKTEIV RVYLIFASEL 

       250        260        270        280        290        300 
AVNYNHQLNV HMQEILPKLC EYHDEDAFRD DTRNLFFQCV SKSLHSMYLK MDMCDFNTLG 

       310        320        330        340        350        360 
VPVHEKWPQT LLRLKTIVNV EIRKNSWARC KNALLSNNKF SDPFIKMSAL AMYIVLWHLE 

       370        380        390        400        410        420 
TKKADENGEG DAPKKIPKPA DKMETIFSLI DKKENTFNDV WLAIFTEILQ LSSVILNVAN 

       430        440        450        460        470        480 
YQMALTTVAE IMQMYGNAKN LRNLRLCLAH LLTKEQELLH SKSIREDFLG ELWSQMANQL 

       490        500        510        520        530        540 
ISETTTNSEE IKEKQLVLQM LIRHNKLNQK LSSTLLNNII SNEMLKRNEC LATIREIFIH 

       550        560        570        580        590        600 
ADKCGQDKAS ADLEPIIAWA YGSADRFIAA QMIHNIDSID AQLQADTFAI SIINFLDVQQ 

       610        620        630        640        650        660 
LRQISQSEHI VPSTERNLLA YKYNEQLICF DKDYATPFES ITHIQSETKN CLIQSNYDCL 

       670        680        690        700        710        720 
MRGLNFEIAK ENKPAAIIKN LNSLLKLICT MERLLHYKVF DADTYTGCPL IKRIGLYLSH 

       730        740        750        760        770        780 
IEFQLKANGA EILDKSDLPE ILRLEIYVLD VFRTNLVLLD YLERQPIEML VEFVGAALKL 

       790        800        810        820        830        840 
HSMQRERSVD ADHGTITRLC LNILAGLCAY NSHRDEAFEH IVKVTMRWHP QDVLIVTKML 

       850        860        870        880        890        900 
CSCQTISEAS SSWLVSKLKT LFQQHHQDAE LMDKVVQHMP TIFYFVRHKE NHLDDMLMAL 

       910        920        930        940        950        960 
NSLLRIAIKK SYTSNLTAKI VRCVGLIAQR CPDIYLLENF AVICKSTAKF ITMPTLEVRF 

       970        980        990       1000       1010       1020 
ATLFTFTILL ESNCVTSDAI GHSRTHWDFC QELYESIEFK KLTYNNEDAI QNSNALIVQM 

      1030       1040       1050       1060       1070       1080 
LIAIFLRSSF HQEIALKELL HHCALRRLTE REFISLQSMS SCHRQTVRDL IRPFAAILLH 

      1090       1100       1110       1120       1130       1140 
HWSSKRWPIS KFPYFLCYST KSEFRKTHAS EIMAYTFLYG KTEDIERCSK SISEELALPI 

      1150       1160       1170       1180       1190       1200 
LASFLLIKNS SCSESEGQNF KEHLQLLSEN LSYSQLNATD VDLDLDILCY VISMLHDPQE 

      1210       1220       1230       1240       1250       1260 
MMRLFGSLAI CNRTASWYSL SGESLFKCLN FHIDPEMRPS MDSRIQSMTT LQTKHSRVLV 

      1270       1280       1290       1300       1310       1320 
DIFGRLKTNC YSASFSSQAL HDFFLYCEVA DAVYDAARKN ETIVTQCSFF VRDIWFFVVR 

      1330       1340       1350       1360       1370       1380 
LLIHTKFIRV QMAALTFLEL LLNKHNFRLD DYQNHFGDIA KLLSNFQLCC EAKEVREKTM 

      1390       1400       1410       1420       1430       1440 
SIVMYILESK KGHINLNSFL EETTDCEFLK PLREDCKSSQ PNIDRADVAN YLRSFLLSPT 

      1450       1460       1470       1480       1490       1500 
PERLRDLRTY IAEHKDKVQE HEKLLFGVIN KLIQMTRDAH NKTTSIDSLK CLAQIGPLKI 

      1510       1520       1530       1540       1550       1560 
STVSYYFQTD FEAFEKSNGE PMEAFLGVVC HSLDTSLFQF DPKTHEGLVS VAIQVVNSKP 

      1570       1580       1590       1600       1610       1620 
GSNIMERYKN LRIFADKSTA STFLHSNKQI RRIDWLSILK ATKSLSYEPW MCAFVSKVFQ 

      1630       1640       1650       1660       1670       1680 
MCGWLGFDKL AATSFAFAKT CLLPFIKLLL ENSLEHVESL SQMLDYFFEG FTSSTAPNSQ 

      1690       1700       1710       1720       1730       1740 
EIFRNKRAIK KFLHICEYIR IFNNWTIPIN LSNVVMASNH CQAYFLSIMY LELWACSESP 

      1750       1760       1770       1780       1790       1800 
KSKADFLDNE CFQDGAKKAY ESIGCLDAIP GFVNPMRSRL DFLGHGSNLS TILLESDHLD 

      1810       1820       1830       1840       1850       1860 
RASGQLCIDI MKGNGLWSFA KLQQHQNIEP DYEIFWRLGQ WDSLTDPKHQ QNQTVVRTSL 

      1870       1880       1890       1900       1910       1920 
DLEQEFKRHH FVALRSIGQR EEENSLSAIE QAYSCVRDIL MEISVECLQS VYKYLTWLCS 

      1930       1940       1950       1960       1970       1980 
LQQAEDFCQI QFGTQLDPAS TTKIFRKWQT ELELKYGNFS CKEYVIAHQI ALLKLAGTRA 

      1990       2000       2010       2020       2030       2040 
SRRMSEFYQK DPISTYLMKG IEECKSAGKL NLAAKYTATL RELPNIRESI KISVLLEDAE 

      2050       2060       2070       2080       2090       2100 
INLKMGNQQI AKAILDYVTN NNEFVYCVQR VPALRMQGEF LLDCNAETLS WVQSHKFNNS 

      2110       2120       2130       2140       2150       2160 
LKLIDDFVQH RQTLSEKYRD IFDWHQLDAY ASKQRTAAYA TIAKYADREY QQLHDYRHSQ 

      2170       2180       2190       2200       2210       2220 
EYQTLKDIIE QNRQTAEKVT QRENQDRRVI SVQMKRYASL DEQQLNQIEE KLTEYLRLAL 

      2230       2240       2250       2260       2270       2280 
TNYMAYCRLD SGFSSAAIYR IISLWFTNAT SKQCQECIKD EILTVPSYKF ICAANQLTAR 

      2290       2300       2310       2320       2330       2340 
LNSKNTSLLK GLTDLLVQCG KDHPYHTFYQ LYPLVFAHLD GENSNTERSG IARKIIAMIC 

      2350       2360       2370       2380       2390       2400 
EKNGTAGECS KQLESLLPAL ITFANEGKTN DNRPVSDSVR NKQFDKVRRW RNLNAVHCPT 

      2410       2420       2430       2440       2450       2460 
LELPVMPSKE YSIISVVKWT NETTQCGGLN APVKIMCVCS DGKIRAQLVK GKDDLRQDAV 

      2470       2480       2490       2500       2510       2520 
MQQVFGIVNE LLNQDSEFIE RKLKLRTYKV TPLSMRSGIL EWCTNSVPVG HYLVVEGKGG 

      2530       2540       2550       2560       2570       2580 
AHARYRPNDW NNNKCRKLSS DHLKSPKETR YAIYKKICEN IKPVFHYFLL EKFPIPGVWF 

      2590       2600       2610       2620       2630       2640 
ERRLAYTNSV ATTSMVGYVL GLGDRHTQNI LVDQQTAEVI HIDFGIAFEQ GKIQTTPETV 

      2650       2660       2670       2680       2690       2700 
PFRLTRDFVA PMGICGTKGV FAKSCEATMH ILRRYKSVFT TILEVLLYDP LFIWGVLKKK 

      2710       2720       2730       2740       2750       2760 
QSPQQSGEES VNLVAQRALL LVQNKLDGRE AGTMGDSNVE AQVERLINEA TLPSNLCMLF 


PGWDPHL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"Drosophila melanogaster MNK/Chk2 and p53 regulate multiple DNA repair and apoptotic pathways following DNA damage." Brodsky M.H., Weinert B.T., Tsang G., Rong Y.S., McGinnis N.M., Golic K.G., Rio D.C., Rubin G.M. Mol. Cell. Biol. 24:1219-1231(2004) [PubMed: 14729967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1569-2767.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2183-2767. Strain: Berkeley. Tissue: Testis.
[5]	"ATM is required for telomere maintenance and chromosome stability during Drosophila development." Silva E., Tiong S., Pedersen M., Homola E., Royou A., Fasulo B., Siriaco G., Campbell S.D. Curr. Biol. 14:1341-1347(2004) [PubMed: 15296750] [Abstract] Cited for: FUNCTION.
[6]	"Telomere protection without a telomerase; the role of ATM and Mre11 in Drosophila telomere maintenance." Bi X., Wei S.-C.D., Rong Y.S. Curr. Biol. 14:1348-1353(2004) [PubMed: 15296751] [Abstract] Cited for: FUNCTION.
[7]	"The Drosophila ATM ortholog, dATM, mediates the response to ionizing radiation and to spontaneous DNA damage during development." Song Y.-H., Mirey G., Betson M., Haber D.A., Settleman J. Curr. Biol. 14:1354-1359(2004) [PubMed: 15296752] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[8]	"Drosophila atm/telomere fusion is required for telomeric localization of HP1 and telomere position effect." Oikemus S.R., McGinnis N., Queiroz-Machado J., Tukachinsky H., Takada S., Sunkel C.E., Brodsky M.H. Genes Dev. 18:1850-1861(2004) [PubMed: 15256487] [Abstract] Cited for: IDENTIFICATION, FUNCTION.
[9]	"Drosophila ATM and Mre11 are essential for the G2/M checkpoint induced by low-dose irradiation." Bi X., Gong M., Srikanta D., Rong Y.S. Genetics 171:845-847(2005) [PubMed: 16020777] [Abstract] Cited for: FUNCTION.
[10]	"Drosophila ATM and ATR checkpoint kinases control partially redundant pathways for telomere maintenance." Bi X., Srikanta D., Fanti L., Pimpinelli S., Badugu R., Kellum R., Rong Y.S. Proc. Natl. Acad. Sci. U.S.A. 102:15167-15172(2005) [PubMed: 16203987] [Abstract] Cited for: FUNCTION.

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Cross-references

Sequence databases
	AE014297 Genomic DNA. Translation: ABI31168.1. AY395750 mRNA. Translation: AAR89513.1. AY113192 mRNA. Translation: AAM29197.1. Different initiation. BK004084 mRNA. Translation: DAA04940.1.
RefSeq	NP_001036712.1.
UniGene	Dm.13357
3D structure databases
HSSP	HSSP built from PDB template 1E8Y based on UniProtKB P48736.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5EAK6.
Genome annotation databases
GeneID	41839.
KEGG	dme:Dmel_CG6535.
Organism-specific databases
FlyBase	FBgn0045035. tefu.
Family and domain databases
InterPro	IPR015519. Ataxia_Telang_Mut. IPR003152. FATC. IPR000403. PI3/4_kinase_cat. IPR014009. PIK_FAT. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
PANTHER	PTHR11139:SF3. Ataxia_Telang_Mut. 1 hit.
Pfam	PF02260. FATC. 1 hit. PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS51189. FAT. 1 hit. PS51190. FATC. 1 hit. PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...
Other Resources
NextBio	825818.

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Entry information

Entry name

ATM_DROME

Accession

Primary (citable) accession number: Q5EAK6
Secondary accession number(s): Q0KI71

Q9VFB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 15, 2005
Last modified:	November 4, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Family and domain databases

Other Resources

Entry information

Relevant documents