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Reviewed, UniProtKB/Swiss-Prot Q4V5A3 (UNPG_DROME)

Last modified November 4, 2008. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Homeobox protein unplugged
Gene names
Name: unpg
Synonyms: unp
ORF Names: CG1650
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Plays a regulatory role in neural branching of the tracheae: segment-specific aspects of these neural branching patterns appear to be generated by homeotic regulation of expression. May have a role with oc/otd in the postembryonic development of the brain.

Subcellular location

Nucleus. Note= In tracheal precursor cells as they migrate and extend.

Tissue specificity

Expressed in the neuroectodermal and mesectodermal cells at the ventral midline of stage 8 embryos, Subsequently, expression domains in the CNS widen and have their most anterior border in the posterior deutocerebrum. Oc/otd and unpg are mutual repressors at the interface of their brain-specific expression domains. Expression fades during germ band retraction and is then restricted to subset of cells by stage 14. Expressed in the founder cells of the cerebral branch within the first tracheal metamere. Outside the CNS, expression is seen in two clusters of ectodermal cells located laterally within the labial and first thoracic segments of stage 9 embryos. By stage 13, the expression is detected in a few cells close to the dorsal midline of the embryos.

Developmental stage

First detected in 4 hours embryos, expression persists throughout embryogenesis, levels drop during larval stages and then increase again during pupal and adult stages.

Miscellaneous

Fly embryos lacking unpg display segmentally repeated ganglionic branches that stall and fail to penetrate the CNS and the segment-specific cerebral branch and associated cerebral anastomosis fail to form.

Sequence similarities

Contains 1 homeobox DNA-binding domain.

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Ontologies

Keywords

   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Technical termComplete proteome

Gene Ontology (GO)

   Biological processbrain development Ref.5

Inferred from mutant phenotype. Source: UniProtKB

regulation of nervous system development Ref.1

Inferred from mutant phenotype. Source: UniProtKB

regulation of transcription Ref.1

Inferred from mutant phenotype. Source: UniProtKB

tracheal outgrowth, open tracheal system

Traceable author statement. Source: FlyBase

   Cellular componentnucleus Ref.1

Inferred from direct assay. Source: UniProtKB

   Molecular functiontranscription factor activity Ref.1

Inferred from mutant phenotype. Source: UniProtKB

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485Homeobox protein unplugged
PRO_0000299499

Regions

DNA binding319 – 37860Homeobox
Compositional bias111 – 16252Pro-rich

Experimental info

Sequence conflict1071G → A in AAA97404. Ref.1
Sequence conflict4811A → P in AAA97404. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q4V5A3-1 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 16B4EF888ADE1D56

FASTA48552,230
        10         20         30         40         50         60 
MERPALLQNG EIGTMESPTT RLASKPFPKP FSIESLIANQ TPATATPPSP PEERDQEQEA 

        70         80         90        100        110        120 
EQEQELSARA MVASSALGLT QFPLYNPWLH GYFAQNHERL THLIAGGCYL PSSPAGHPAA 

       130        140        150        160        170        180 
QQPQAQAQPQ PPPPHPPTHA LEKQLPPTLP HPLDTRFLPF NPAAAGVAPT DLSYRRLAEL 

       190        200        210        220        230        240 
MNQDYVHSLS VHARLQHMAA AGRMHEDQAN PGMAQLQEPT PPQAHSSPAK SGSHSPMEPA 

       250        260        270        280        290        300 
LDVGMDEDFE CSGDSCSDIS LTMSPRNYNG EMDKSRNGAY TNSDSEDCSD DEGAQSRHEG 

       310        320        330        340        350        360 
GGMGGKDSQG NGSSSNSKSR RRRTAFTSEQ LLELEREFHA KKYLSLTERS QIATSLKLSE 

       370        380        390        400        410        420 
VQVKIWFQNR RAKWKRVKAG LTSHGLGRNG TTSGTKIVVP IPVHVNRFAV RSQHQQLEKM 

       430        440        450        460        470        480 
CLSGPKPDLR KKLSAEAIGG FEKFSGSTNA SSPSGGPVGL GVGVGVGVGV GLGVSTPLSL 


ARSIY

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References

« Hide 'large scale' references
[1]"Control of Drosophila tracheal branching by the novel homeodomain gene unplugged, a regulatory target for genes of the bithorax complex."
Chiang C., Young K.E., Beachy P.A.
Development 121:3901-3912(1995) [PubMed: 8582298] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
[4]Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
[5]"An urbilaterian origin of the tripartite brain: developmental genetic insights from Drosophila."
Hirth F., Kammermeier L., Frei E., Walldorf U., Noll M., Reichert H.
Development 130:2365-2373(2003) [PubMed: 12702651] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.

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Cross-references

Sequence databases

U35427 mRNA. Translation: AAA97404.1.
AE013599 Genomic DNA. Translation: AAF58968.1.
BT022753 mRNA. Translation: AAY55169.1.
RefSeqNP_477146.1.
UniGeneDm.6807

3D structure databases

HSSPHSSP built from PDB template 1B72 based on UniProtKB P14653.
ModBaseSearch...

Genome annotation databases

EnsemblCG1650. Drosophila melanogaster. [Contig view]
GeneID35942.
KEGGdme:Dmel_CG1650.
NMPDRfig|7227.3.peg.4041.

Organism-specific databases

FlyBaseFBgn0015561. unpg.

Gene expression databases

ArrayExpressQ4V5A3.

Family and domain databases

InterProIPR001356. Homeobox.
IPR012287. Homeodomain-rel.
[Graphical view]
Gene3DG3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00024. HOMEOBOX.
ProDomPD000010. Homeobox. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
BLOCKSSearch...
ProtoNetSearch...

Other Resources

NextBio795976.

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Entry information

Entry nameUNPG_DROME
AccessionPrimary (citable) accession number: Q4V5A3
Secondary accession number(s): Q24136
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 3, 2006
Last modified: November 4, 2008
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents