Shaggy-related protein kinase eta - Arabidopsis thaliana (Mouse-ear cress)

Names and origin

Protein names

Recommended name:
    Shaggy-related protein kinase eta
    EC=2.7.11.1
Alternative name(s):
    ASK-eta
    Protein BRASSINOSTEROID INSENSITIVE 2
    Protein ULTRACURVATA 1

Gene names

Name:	ASK7
Synonyms:	BIN2, DWF12, UCU1
Ordered Locus Names:	At4g18710
ORF Names:	F28A21.120

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Hide | Top

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Negative regulator in brassinosteroid signal transduction pathway important for plant growth. May be also involved in auxin signaling pathway. Phosphorylates and increases the degradation of BZR1 and BZR2/BES1 by the proteasome. Phosphorylates beet curly top virus C4 and tomato golden mosaic virus AC4 on threonine and serine residues.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Inactivated by an unknown mechanism after binding of brassinosteroids to the brassinosteroid receptor complex. Inhibited by lithium.
Subunit structure	Interacts in vitro with the C-terminal fragment of BZR1 and with BES1/BZR2, but not through the kinase domain. Interacts with beet curly top virus AL4/C4 and tomato golden mosaic virus AL4/AC4.
Tissue specificity	In the two outer cell layers of the developing seed coat and restricted to the suspensor cells in developing embryos.
Post-translational modification	Autophosphorylated mainly on threonine and serine residues.
Miscellaneous	Unlike other GSK3 kinases, does not require a priming phosphorylation event or the presence of a scaffold protein to phosphorylate its substrates.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. Contains 1 protein kinase domain.

Hide | Top

Ontologies

Keywords
Biological process	Host-virus interaction
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-1798250,EBI-1798250
ARF2	Q94JM3	3	EBI-1798250,EBI-1799262
BZR2	Q9LN63	1	EBI-1798250,EBI-617078

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 380

380

Shaggy-related protein kinase eta

PRO_0000086222

Regions

Domain

40 – 324

285

Protein kinase

Nucleotide binding

46 – 54

9

ATP By similarity

Sites

Active site

165

1

Proton acceptor By similarity

Binding site

69

1

ATP By similarity

Amino acid modifications

Modified residue

104

1

Phosphothreonine Potential

Modified residue

105

1

Phosphoserine Potential

Modified residue

187

1

Phosphoserine Potential

Modified residue

220

1

Phosphothreonine Potential

Modified residue

261

1

Phosphothreonine Potential

Modified residue

310

1

Phosphoserine Potential

Modified residue

314

1

Phosphothreonine Potential

Modified residue

353

1

Phosphoserine Potential

Experimental info

Mutagenesis

69

1

K → R: No kinase activity

Mutagenesis

80

1

R → A: No effect

Mutagenesis

261

1

T → I in bin2-2; brassinosteroid-insensitive dwarf mutant; increased kinase activity

Mutagenesis

263

1

E → K in dwf12-2D/bin2-1; brassinosteroid-insensitive dwarf mutant; increased kinase activity

Mutagenesis

264

1

E → K in dwf12-1D/ucu1-1/ucu1-2; brassinosteroid-insensitive dwarf mutant

Mutagenesis

284

1

P → S in ucu1-3; leaves rolled spirally downward

Sequence conflict

105

1

S → E Ref.1 Ref.2

Sequence conflict

121

1

S → T Ref.1 Ref.2

Sequence conflict

145

1

M → S Ref.1 Ref.2

Sequence conflict

157 – 159

3

NVA → SCP Ref.1 Ref.2

Sequence conflict

196

1

A → P Ref.1 Ref.2

Sequence conflict

271

1

H → N Ref.1 Ref.2

Sequence conflict

276

1

R → K Ref.1 Ref.2

Sequence conflict

298 – 300

3

IDF → VDL Ref.1 Ref.2

Sequence conflict

327

1

L → H in AAL77705. Ref.7

Sequence conflict

340

1

F → L Ref.1 Ref.2

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q39011-1 [UniParc].

Last modified May 10, 2004. Version 2.
Checksum: 86C2FD5F77201168
Show »

FASTA

380

43,099

        10         20         30         40         50         60 
MADDKEMPAA VVDGHDQVTG HIISTTIGGK NGEPKQTISY MAERVVGTGS FGIVFQAKCL 

        70         80         90        100        110        120 
ETGETVAIKK VLQDRRYKNR ELQLMRVMDH PNVVCLKHCF FSTTSKDELF LNLVMEYVPE 

       130        140        150        160        170        180 
SLYRVLKHYS SANQRMPLVY VKLYMYQIFR GLAYIHNVAG VCHRDLKPQN LLVDPLTHQV 

       190        200        210        220        230        240 
KICDFGSAKQ LVKGEANISY ICSRFYRAPE LIFGATEYTT SIDIWSAGCV LAELLLGQPL 

       250        260        270        280        290        300 
FPGENAVDQL VEIIKVLGTP TREEIRCMNP HYTDFRFPQI KAHPWHKIFH KRMPPEAIDF 

       310        320        330        340        350        360 
ASRLLQYSPS LRCTALEACA HPFFDELREP NARLPNGRPF PPLFNFKQEV AGSSPELVNK 

       370        380 
LIPDHIKRQL GLSFLNQSGT

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three new cDNAs related to SGG/GSK-3 (SHAGGY/glycogen synthase kinase-3) from Arabidopsis thaliana." Dornelas M.C., Schwebel-Dugue N., Thomas M., Lecharny A., Kreis M. (er) Plant Gene Register PGR97-008 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"The Arabidopsis SHAGGY-related protein kinase (ASK) gene family: structure, organization and evolution." Dornelas M.C., Lejeune B., Dron M., Kreis M. Gene 212:249-257(1998) [PubMed: 9611268] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Arabidopsis brassinosteroid-insensitive dwarf12 mutants are semidominant and defective in a glycogen synthase kinase 3beta-like kinase." Choe S., Schmitz R.J., Fujioka S., Takatsuto S., Lee M.-O., Yoshida S., Feldmann K.A., Tax F.E. Plant Physiol. 130:1506-1515(2002) [PubMed: 12428015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANTS DWF12.
[4]	"The UCU1 Arabidopsis gene encodes a SHAGGY/GSK3-like kinase required for cell expansion along the proximodistal axis." Perez-Perez J.M., Ponce M.R., Micol J.L. Dev. Biol. 242:161-173(2002) [PubMed: 11820813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANTS UCU1.
[5]	"Regulation of brassinosteroid signaling by a GSK3/SHAGGY-like kinase." Li J., Nam K.H. Science 295:1299-1301(2002) [PubMed: 11847343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTANTS LYS-69 AND BIN2.
[6]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[7]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[8]	"Arabidopsis ORF clone." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[9]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[10]	"Characterization of three novel members of the Arabidopsis SHAGGY-related protein kinase (ASK) multigene family." Dornelas M.C., Wittich P., von Recklinghausen I., van Lammeren A., Kreis M. Plant Mol. Biol. 39:137-147(1999) [PubMed: 10080716] [Abstract] Cited for: TISSUE SPECIFICITY.
[11]	"Two putative BIN2 substrates are nuclear components of brassinosteroid signaling." Zhao J., Peng P., Schmitz R.J., Decker A.D., Tax F.E., Li J. Plant Physiol. 130:1221-1229(2002) [PubMed: 12427989] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ARG-80, INTERACTION WITH BZR1 AND BZR2/BES1.
[12]	"The GSK3-like kinase BIN2 phosphorylates and destabilizes BZR1, a positive regulator of the brassinosteroid signaling pathway in Arabidopsis." He J.-X., Gendron J.M., Yang Y., Li J., Wang Z.-Y. Proc. Natl. Acad. Sci. U.S.A. 99:10185-10190(2002) [PubMed: 12114546] [Abstract] Cited for: FUNCTION, INTERACTION WITH BZR1.
[13]	"Geminivirus pathogenicity protein C4 interacts with Arabidopsis thaliana shaggy-related protein kinase AtSKeta, a component of the brassinosteroid signalling pathway." Piroux N., Saunders K., Page A., Stanley J. Virology 362:428-440(2007) [PubMed: 17280695] [Abstract] Cited for: FUNCTION, INTERACTION WITH BEET CURLY TOP VIRUS AL4 AND TOMATO GOLDEN MOSAIC VIRUS AL4, AUTOPHOSPHORYLATION.

Hide | Top

Cross-references

Sequence databases
	X94939 mRNA. Translation: CAA64409.1. Y08947 Genomic DNA. Translation: CAA70144.1. AY157149 Genomic DNA. Translation: AAN71719.1. AL035526 Genomic DNA. Translation: CAB37456.1. AL161549 Genomic DNA. Translation: CAB78873.1. AY075699 mRNA. Translation: AAL77705.1. BT026031 mRNA. Translation: ABG48387.1. AY086529 mRNA. Translation: AAM63594.1.
PIR	T04863.
RefSeq	NP_193606.1.
UniGene	At.22875
3D structure databases
HSSP	HSSP built from PDB template 1I09 based on UniProtKB P49841.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q39011.
Genome annotation databases
GeneID	827605.
GenomeReviews	Gene locus AT4G18710 in contig CT486007_GR.
KEGG	ath:AT4G18710.
NMPDR	fig\|3702.1.peg.19677.
Organism-specific databases
GeneFarm	589. 93.
TAIR	At4g18710.
Gene expression databases
ArrayExpress	Q39011.
GermOnline	AT4G18710. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Hide | Top

Entry information

Entry name

KSG7_ARATH

Accession

Primary (citable) accession number: Q39011
Secondary accession number(s): Q147R2, Q8S9H8, Q9SN42

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 10, 2004
Last modified:	November 4, 2008
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents