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Reviewed, UniProtKB/Swiss-Prot Q9VUQ5 (AGO2_DROME)

Last modified July 22, 2008. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Protein argonaute-2
Gene names
Name: AGO2
ORF Names: CG7439
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length1214 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Essential for RNA interference (RNAi); double-stranded RNA induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger.

Subunit structure

Interacts with Fmr1 to form a ribonucleoprotein (RNP) complex involved in translation regulation, other components of the complex are mRpL5, mRpL11, Rm62 and Dcr-1. Interacts with Dcr-1 to form components of the RISC.

Sequence similarities

Belongs to the argonaute family.

Contains 1 PAZ domain.

Contains 1 Piwi domain.

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Ontologies

Keywords

   Biological processRNA-mediated gene silencing
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandRNA-binding
   Technical term3D-structure
Complete proteome
Direct protein sequencing

Gene Ontology (GO)

   Biological processRNA interference, production of siRNA Ref.6

Inferred from direct assay. Source: UniProtKB

   Molecular functionprotein binding Ref.6 Ref.7

Inferred from physical interaction. Source: UniProtKB

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

Q9VJZ11EBI-442476,EBI-149609
Dcr-1Q9VCU91EBI-442476,EBI-112170
Fmr1Q9NFU02EBI-442476,EBI-422631

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform B (identifier: Q9VUQ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform C (identifier: Q9VUQ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MGKKDK → MHFPITTPE
Notes: No experimental confirmation available.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 12141214Protein argonaute-2

Regions

Domain608 – 717110PAZ
Domain885 – 1186302Piwi
Compositional bias19 – 391373Gln/Gly-rich

Natural variations

Alternative sequence1 – 66MGKKDK → MHFPITTPE in isoform C.
Natural variant7141S → R in strain: 186.
Natural variant7401S → P in strain: 130.
Natural variant7831S → N in strain: 138, 140 and 196.
Natural variant8351T → A in strain: 128, 130, 138, 140, 141, 186, 187 and 196.
Natural variant8541S → T in strain: 128, 130, 138, 140, 141, 186, 187 and 196.
Natural variant8661D → E in strain: 128, 130, 141, 186 and 187.

Experimental info

Sequence conflict2011G → D in AAO39550. Ref.3

Secondary structure

1 ............................ 1214
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform B [UniParc].

Last modified August 16, 2004. Version 3.
Checksum: 0DC73AF09CC33F73

FASTA1,214136,850
        10         20         30         40         50         60 
MGKKDKNKKG GQDSAAAPQP QQQQKQQQQR QQQPQQLQQP QQLQQPQQLQ QPQQQQQQQP 

        70         80         90        100        110        120 
HQQQQQSSRQ QPSTSSGGSR ASGFQQGGQQ QKSQDAEGWT AQKKQGKQQV QGWTKQGQQG 

       130        140        150        160        170        180 
GHQQGRQGQD GGYQQRPPGQ QQGGHQQGRQ GQEGGYQQRP PGQQQGGHQQ GRQGQEGGYQ 

       190        200        210        220        230        240 
QRPSGQQQGG HQQGRQGQEG GYQQRPPGQQ QGGHQQGRQG QEGGYQQRPS GQQQGGHQQG 

       250        260        270        280        290        300 
RQGQEGGYQQ RPPGQQQGGH QQGRQGQEGG YQQRPPGQQQ GGHEQGRQGQ EGGYQQRPSG 

       310        320        330        340        350        360 
QQQGGHQQGR QGQEGGYQQR PSGQQQGGHQ QGRQGQEGGY QQRPSGQQQG GHQQGRQGQE 

       370        380        390        400        410        420 
GGYQQRPPGQ QPNQTQSQGQ YQSRGPPQQQ QAAPLPLPPQ PAGSIKRGTI GKPGQVGINY 

       430        440        450        460        470        480 
LDLDLSKMPS VAYHYDVKIM PERPKKFYRQ AFEQFRVDQL GGAVLAYDGK ASCYSVDKLP 

       490        500        510        520        530        540 
LNSQNPEVTV TDRNGRTLRY TIEIKETGDS TIDLKSLTTY MNDRIFDKPM RAMQCVEVVL 

       550        560        570        580        590        600 
ASPCHNKAIR VGRSFFKMSD PNNRHELDDG YEALVGLYQA FMLGDRPFLN VDISHKSFPI 

       610        620        630        640        650        660 
SMPMIEYLER FSLKAKINNT TNLDYSRRFL EPFLRGINVV YTPPQSFQSA PRVYRVNGLS 

       670        680        690        700        710        720 
RAPASSETFE HDGKKVTIAS YFHSRNYPLK FPQLHCLNVG SSIKSILLPI ELCSIEEGQA 

       730        740        750        760        770        780 
LNRKDGATQV ANMIKYAATS TNVRKRKIMN LLQYFQHNLD PTISRFGIRI ANDFIVVSTR 

       790        800        810        820        830        840 
VLSPPQVEYH SKRFTMVKNG SWRMDGMKFL EPKPKAHKCA VLYCDPRSGR KMNYTQLNDF 

       850        860        870        880        890        900 
GNLIISQGKA VNISLDSDVT YRPFTDDERS LDTIFADLKR SQHDLAIVII PQFRISYDTI 

       910        920        930        940        950        960 
KQKAELQHGI LTQCIKQFTV ERKCNNQTIG NILLKINSKL NGINHKIKDD PRLPMMKNTM 

       970        980        990       1000       1010       1020 
YIGADVTHPS PDQREIPSVV GVAASHDPYG ASYNMQYRLQ RGALEEIEDM FSITLEHLRV 

      1030       1040       1050       1060       1070       1080 
YKEYRNAYPD HIIYYRDGVS DGQFPKIKNE ELRCIKQACD KVGCKPKICC VIVVKRHHTR 

      1090       1100       1110       1120       1130       1140 
FFPSGDVTTS NKFNNVDPGT VVDRTIVHPN EMQFFMVSHQ AIQGTAKPTR YNVIENTGNL 

      1150       1160       1170       1180       1190       1200 
DIDLLQQLTY NLCHMFPRCN RSVSYPAPAY LAHLVAARGR VYLTGTNRFL DLKKEYAKRT 

      1210 
IVPEFMKKNP MYFV

« Hide

Isoform C [UniParc].

Checksum: 7C794ABF4D993EAF
Show »

1,217137,216

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References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[3]Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. expand/collapse author list , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
Strain: Berkeley.
Tissue: Embryo.
[4]"Natural selection drives extremely rapid evolution in antiviral RNAi genes."
Obbard D.J., Jiggins F.M., Halligan D.L., Little T.J.
Curr. Biol. 16:580-585(2006) [PubMed: 16546082] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 376-1214, VARIANTS ARG-714; PRO-740; ASN-783; ALA-835; THR-854 AND GLU-866.
Strain: 128, 130, 138, 140, 141, 186, 187 and 196.
[5]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 449-1214, VARIANTS ASN-783; ALA-835 AND THR-854.
Strain: Berkeley.
Tissue: Ovary.
[6]"Argonaute2, a link between genetic and biochemical analyses of RNAi."
Hammond S.M., Boettcher S., Caudy A.A., Kobayashi R., Hannon G.J.
Science 293:1146-1150(2001) [PubMed: 11498593] [Abstract]
Cited for: PROTEIN SEQUENCE OF 506-515; 525-531; 554-557; 617-627; 662-674; 809-815 AND 1181-1188 (ISOFORM B), FUNCTION, INTERACTION WITH DCR-1.
[7]"A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins."
Ishizuka A., Siomi M.C., Siomi H.
Genes Dev. 16:2497-2508(2002) [PubMed: 12368261] [Abstract]
Cited for: INTERACTION WITH FMR1.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AE014296 Genomic DNA. Translation: AAF49619.2.
AE014296 Genomic DNA. Translation: AAF49620.2.
BT003546 mRNA. Translation: AAO39550.1.
DQ228766 Genomic DNA. Translation: ABB54719.1.
DQ228767 Genomic DNA. Translation: ABB54720.1.
DQ228768 Genomic DNA. Translation: ABB54721.1.
DQ228769 Genomic DNA. Translation: ABB54722.1.
DQ228770 Genomic DNA. Translation: ABB54723.1.
DQ228771 Genomic DNA. Translation: ABB54724.1.
DQ228772 Genomic DNA. Translation: ABB54725.1.
DQ228773 Genomic DNA. Translation: ABB54726.1.
AY094751 mRNA. Translation: AAM11104.1. Different initiation.
RefSeqNP_648775.1.
NP_730054.1.
UniGeneDm.9677

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1R6ZX-ray2.80A/P/Z591-726[»]
1T2RNMR-A602-720[»]
1T2SNMR-A602-720[»]
1VYNNMR-A602-740[»]
SMRQ9VUQ5. Positions 591-719.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9VUQ5.

Genome annotation databases

EnsemblCG7439. Drosophila melanogaster. [Contig view]
GeneID39683.
KEGGdme:Dmel_CG7439.

Organism-specific databases

FlyBaseFBgn0087035. AGO2.

Gene expression databases

ArrayExpressQ9VUQ5.
GermOnlineCG7439. Drosophila melanogaster.

Family and domain databases

InterProIPR014811. DUF1785.
IPR003100. PAZ.
IPR003165. Piwi.
[Graphical view]
PfamPF08699. DUF1785. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
PROSITEPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProDomQ9VUQ5.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

LinkHubQ9VUQ5.
ProtoNetSearch...

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Entry information

Entry nameAGO2_DROME
AccessionPrimary (citable) accession number: Q9VUQ5
Secondary accession number(s): Q2Q3Y0 expand/collapse secondary AC list , Q2Q3Y1, Q2Q3Y3, Q2Q3Y4, Q2Q3Y5, Q86P07, Q8T3N2, Q9VUQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: July 22, 2008
This is version 55 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project