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Reviewed, UniProtKB/Swiss-Prot Q26454 (MCM4_DROME)

Last modified November 4, 2008. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA replication licensing factor MCM4
Alternative name(s):
    Protein disc proliferation abnormal
Gene names
Name: dpa
ORF Names: CG1616
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length866 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Essential role in mitotic DNA replication but not in endoreplication.

Subcellular location

NucleusBy similarity.

Sequence similarities

Belongs to the MCM family.

Contains 1 MCM domain.

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Ontologies

Keywords

   Biological processDNA replication
Transcription
Transcription regulation
   Cellular componentNucleus
   LigandATP-binding
DNA-binding
Nucleotide-binding
   PTMPhosphoprotein
   Technical termComplete proteome

Gene Ontology (GO)

   Biological processDNA replication

Inferred from mutant phenotype. Source: FlyBase

mitotic spindle organization

Inferred from mutant phenotype. Source: FlyBase

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

Mcm6Q9V4611EBI-175772,EBI-869161

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 866866DNA replication licensing factor MCM4
PRO_0000194104

Regions

Domain460 – 669210MCM
Nucleotide binding512 – 5198ATP Potential

Amino acid modifications

Modified residue551Phosphoserine
Modified residue811Phosphoserine
Modified residue871Phosphothreonine

Experimental info

Sequence conflict4351D → E in AAB35644. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q26454-1 [UniParc].

Last modified November 2, 2001. Version 2.
Checksum: CF6ED69D2ADDA04E

FASTA86696,610
        10         20         30         40         50         60 
MSSPARSPSV GGATPKQGAR TPTRGIASQD VETPMRMGPG RAVRPSDNIS LPPTSPGNIS 

        70         80         90        100        110        120 
LPATSPARGL GANMSEIDLS SPLNYGTPSS MGSIRTPRSG IRGTPLRARP DIRTDKRIRQ 

       130        140        150        160        170        180 
VAIGGGSGLE PIPEKGSETT DPVSESSQAP QLVVWGTNVV VSQCKSKFKS FIMRFIDPSA 

       190        200        210        220        230        240 
EQDEISENID VNQPLYLQKL EEIHTLEEPY LNLNCAHLKT FDQDLYRQLI CYPQEVIPGF 

       250        260        270        280        290        300 
DMAINEMFFE RYPAALLEHQ IQVRPFNADK TRNMRSLNPE DMDQLISISG MVIRSSNVIP 

       310        320        330        340        350        360 
EMREAFFSCN ICSFSTTVEV DRGRINQPTL CTNCNTNHCF RLIHNRSEFT DKQLVKLQES 

       370        380        390        400        410        420 
PDDMAAGQTP HNVLLYAHND LVDKVQPGDR VTVTGIYRAT PLKTGGLSSS VKSVYKTHVD 

       430        440        450        460        470        480 
VVHFRKVDNK RLYEDEEGKD HIFPPERVEL LQLLAKKPDI YDRLARAIAP SIYENDDIKK 

       490        500        510        520        530        540 
GILLQLFGGT KKKHATLGRQ NFRSEIHLLL CGDPGTSKSQ MLQYVFNLVP RSQYTSGRGS 

       550        560        570        580        590        600 
SAVGLTAYVT KDPETRQLVL QTGALVLADN GVCCIDEFDK MNDSTRSVLH EVMEQQTLSI 

       610        620        630        640        650        660 
AKAGIICQLN ARTSILAAAN PAESQWNKRK NIIDNVQLPH TLLSRFDLIF LVLDPQDEIF 

       670        680        690        700        710        720 
DKRLASHLVS LYYVTRHEEE DTMFDMSVLR DYIAYAREHL SPTLSDEAQQ RLIQAYVDMR 

       730        740        750        760        770        780 
KVGAGRGQIS AYPRQLESLI RLSEAHAKVR LSNQVELLDV EEAWRLHREA LKQSATDPLS 

       790        800        810        820        830        840 
GKIDVGILTT GLSTAARKKR ADLVAAIKEN LKKKGKVLTV PYQKLFSDIK EGSQIMITRE 

       850        860 
QFEDALKEVQ DEGAIVVMGK NTIRIC

« Hide

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References

« Hide 'large scale' references
[1]"dpa, a member of the MCM family, is required for mitotic DNA replication but not endoreplication in Drosophila."
Feger G., Vaessin H., Su T.T., Wolff E., Jan L.Y., Jan Y.N.
EMBO J. 14:5387-5398(1995) [PubMed: 7489728] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
[4]"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55, MASS SPECTROMETRY.
[5]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; SER-81 AND THR-87, MASS SPECTROMETRY.
Tissue: Embryo.

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Cross-references

Sequence databases

S80255 mRNA. Translation: AAB35644.1.
AE013599 Genomic DNA. Translation: AAF59242.1.
PIRS59872.
RefSeqNP_477185.1.
UniGeneDm.21562

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ26454.

Genome annotation databases

EnsemblCG1616. Drosophila melanogaster. [Contig view]
GeneID35679.
KEGGdme:Dmel_CG1616.
NMPDRfig|7227.3.peg.3663.

Organism-specific databases

FlyBaseFBgn0015929. dpa.

Phylogenomic databases

HOGENOMQ26454.

Enzyme and pathway databases

BioCycDMEL-XXX-02:DMEL-XXX-02-003226-MON.

Gene expression databases

ArrayExpressQ26454.
GermOnlineCG1616. Drosophila melanogaster.

Family and domain databases

InterProIPR003593. AAA+_ATPase_core.
IPR001208. MCM.
IPR008047. MCM_4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHERPTHR11630. MCM. 1 hit.
PfamPF00493. MCM. 1 hit.
[Graphical view]
PRINTSPR01657. MCMFAMILY.
PR01660. MCMPROTEIN4.
ProDomPD001041. MCM. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
PROSITEPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
BLOCKSSearch...
ProtoNetSearch...

Other Resources

NextBio794682.

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Entry information

Entry nameMCM4_DROME
AccessionPrimary (citable) accession number: Q26454
Secondary accession number(s): Q9V4M5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 2, 2001
Last modified: November 4, 2008
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents