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Reviewed, UniProtKB/Swiss-Prot Q24323 (SEM2A_DROME)

Last modified November 4, 2008. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Semaphorin-2A
Alternative name(s):
    Semaphorin-II
      Short name=Sema II
Gene names
Name: Sema-2a
Synonyms: dSema-II
ORF Names: CG4700
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length724 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Plays a role in growth cones guidance. Required for both proper adult behavior and survival. Can function in vivo as a selective target-derived signal that inhibits the formation of specific synaptic terminal arbors.

Subunit structure

Interacts with PLEXB.

Subcellular location

SecretedPotential.

Tissue specificity

Transiently expressed by a single large muscle during motoneuron outgrowth and synapse formation.

Developmental stage

Expression begins around stage 10 in weak epidermal stripes. Nervous system expression is first detected around stage 15 and at stage 16 appears to remain restricted to a small subset of neurons. Beginning around stage 14, it is also expressed at a very high level in a single ventral thoracic muscle fiber in segment T3. It is also expressed in the embryonic gonads and in anterior sensory organs, including the maxillary complex.

Sequence similarities

Belongs to the semaphorin family.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Sema domain.

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Ontologies

Keywords

   Biological processDifferentiation
Neurogenesis
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainImmunoglobulin domain
Signal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
   Technical termComplete proteome

Gene Ontology (GO)

   Biological processaxon guidance

Inferred from mutant phenotype. Source: FlyBase

drinking behavior Ref.1

Inferred from mutant phenotype. Source: FlyBase

flight behavior Ref.1

Inferred from mutant phenotype. Source: FlyBase

synaptic target inhibition

Inferred from mutant phenotype. Source: FlyBase

visual behavior Ref.1

Inferred from mutant phenotype. Source: FlyBase

   Molecular functionprotein binding Ref.6

Inferred from physical interaction. Source: UniProtKB

Complete GO annotation...

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q24323-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Notes: No experimental confirmation available.
Isoform C (identifier: Q24323-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: MSLLQLSPLL...GNNNYGKHGA → MNGIHWLLFGSCLMLISQIEAVTEELSP
Notes: No experimental confirmation available.
Isoform D (identifier: Q24323-3)

The sequence of this isoform differs from the canonical sequence as follows:
     103-125: Missing.
Notes: No experimental confirmation available.
Isoform E (identifier: Q24323-4)

The sequence of this isoform differs from the canonical sequence as follows:
     110-127: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 724699Semaphorin-2A
PRO_0000032301

Regions

Domain45 – 522478Sema
Domain552 – 663112Ig-like C2-type

Amino acid modifications

Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1631N-linked (GlcNAc...) Potential
Glycosylation1901N-linked (GlcNAc...) Potential
Glycosylation2291N-linked (GlcNAc...) Potential
Glycosylation3141N-linked (GlcNAc...) Potential
Glycosylation4011N-linked (GlcNAc...) Potential
Glycosylation5631N-linked (GlcNAc...) Potential
Glycosylation6581N-linked (GlcNAc...) Potential
Glycosylation6701N-linked (GlcNAc...) Potential
Glycosylation7081N-linked (GlcNAc...) Potential
Disulfide bond590 ↔ 647 By similarity

Natural variations

Alternative sequence1 – 5555MSLLQ…GKHGA → MNGIHWLLFGSCLMLISQIE AVTEELSP in isoform C.
VSP_016075
Alternative sequence103 – 12523Missing in isoform D.
VSP_016076
Alternative sequence110 – 12718Missing in isoform E.
VSP_016077

Experimental info

Sequence conflict1051V → A in AAC37187. Ref.1
Sequence conflict1101P → S in AAR99105. Ref.5
Sequence conflict7011A → G in AAC37187. Ref.1
Sequence conflict7111S → C in AAC37187. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Isoform A (B) [UniParc].

Last modified November 8, 2005. Version 2.
Checksum: 600DB0AEA82E677E

FASTA72482,998
        10         20         30         40         50         60 
MSLLQLSPLL ALLLLLCSSV SETAADYENT WNFYYERPCC TGNDQGNNNY GKHGADHVRE 

        70         80         90        100        110        120 
FNCGKLYYRT FHMNEDRDTL YVGAMDRVFR VNLQNISSSN CNRDVINLEP TRDDVVSCVS 

       130        140        150        160        170        180 
KGKSQIFDCK NHVRVIQSMD QGDRLYVCGT NAHNPKDYVI YANLTHLPRS EYVIGVGLGI 

       190        200        210        220        230        240 
AKCPYDPLDN STAIYVENGN PGGLPGLYSG TNAEFTKADT VIFRTDLYNT SAKRLEYKFK 

       250        260        270        280        290        300 
RTLKYDSKWL DKPNFVGSFD IGEYVYFFFR ETAVEYINCG KAVYSRIARV CKKDVGGKNL 

       310        320        330        340        350        360 
LAHNWATYLK ARLNCSISGE FPFYFNEIQS VYQLPSDKSR FFATFTTSTN GLIGSAVCSF 

       370        380        390        400        410        420 
HINEIQAAFN GKFKEQSSSN SAWLPVLNSR VPEPRPGTCV NDTSNLPDTV LNFIRSHPLM 

       430        440        450        460        470        480 
DKAVNHEHNN PVYYKRDLVF TKLVVDKIRI DILNQEYIVY YVGTNLGRIY KIVQYYRNGE 

       490        500        510        520        530        540 
SLSKLLDIFE VAPNEAIQVM EISQTRKSLY IGTDHRIKQI DLAMCNRRYD NCFRCVRDPY 

       550        560        570        580        590        600 
CGWDKEANTC RPYELDLLQD VANETSDICD SSVLKKKIVV TYGQSVHLGC FVKIPEVLKN 

       610        620        630        640        650        660 
EQVTWYHHSK DKGRYEIRYS PTKYIETTER GLVVVSVNEA DGGRYDCHLG GSLLCSYNIT 

       670        680        690        700        710        720 
VDAHRCTPPN KSNDYQKIYS DWCHEFEKYK TAMKSWEKKQ AQCSTRQNFS SNQHPNEIFR 


KPNV

« Hide

Isoform C [UniParc].

Checksum: 6E8321694556D24B
Show »

69780,030
Isoform D [UniParc].

Checksum: 1F6C577FF7144CAC
Show »

70180,470
Isoform E [UniParc].

Checksum: 57159ACA90818E93
Show »

70681,050

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References

« Hide 'large scale' references
[1]"The semaphorin genes encode a family of transmembrane and secreted growth cone guidance molecules."
Kolodkin A.L., Matthes D.J., Goodman C.S.
Cell 75:1389-1399(1993) [PubMed: 8269517] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM E), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Berkeley.
Tissue: Embryo.
[5]Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Berkeley.
Tissue: Embryo.
[6]"Drosophila Plexin B is a Sema-2a receptor required for axon guidance."
Ayoob J.C., Terman J.R., Kolodkin A.L.
Development 133:2125-2135(2006) [PubMed: 16672342] [Abstract]
Cited for: INTERACTION WITH PLEXB.
+Additional computationally mapped references.

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Cross-references

Sequence databases

L26083 mRNA. Translation: AAC37187.1.
AE013599 Genomic DNA. Translation: AAF57989.1.
AE013599 Genomic DNA. Translation: AAM68500.1.
AE013599 Genomic DNA. Translation: AAS64837.1.
AY095026 mRNA. Translation: AAM11354.1.
AY128437 mRNA. Translation: AAM75030.1.
BT011447 mRNA. Translation: AAR99105.1.
PIRC49423.
RefSeqNP_477507.1.
NP_599130.1.
NP_725586.1.
NP_995856.1.
UniGeneDm.14046

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ24323.

Genome annotation databases

EnsemblCG4700. Drosophila melanogaster. [Contig view]
GeneID36846.
KEGGdme:Dmel_CG4700.

Organism-specific databases

FlyBaseFBgn0011260. Sema-2a.

Phylogenomic databases

HOGENOMQ24323.

Gene expression databases

ArrayExpressQ24323.
GermOnlineCG4700. Drosophila melanogaster.

Family and domain databases

InterProIPR007110. Ig-like.
IPR003599. Ig_sub.
IPR003659. Plexin-like.
IPR001627. Sema.
IPR015943. WD40/YVTN_repeat-like.
[Graphical view]
Gene3DG3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PfamPF01403. Sema. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]
BLOCKSSearch...
ProtoNetSearch...

Other Resources

NextBio800670.

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Entry information

Entry nameSEM2A_DROME
AccessionPrimary (citable) accession number: Q24323
Secondary accession number(s): Q0E954 expand/collapse secondary AC list , Q6NN47, Q7KRC9, Q8MLF1, Q9V7Q7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 8, 2005
Last modified: November 4, 2008
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents