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Reviewed, UniProtKB/Swiss-Prot Q24319 (OST48_DROME)

Last modified November 4, 2008. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
      Short name=Oligosaccharyl transferase 48 kDa subunit
      Short name=DDOST 48 kDa subunit
    EC=2.4.1.119
Alternative name(s):
    OST5OP
    DmOST50
      Short name=DrOST
Gene names
Name: Ost48
Synonyms: OST50
ORF Names: CG9022
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.

Catalytic activity

Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane proteinPotential.

Sequence similarities

Belongs to the DDOST 48 kDa subunit family.

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Ontologies

Keywords

   Cellular componentEndoplasmic reticulum
Membrane
   DomainSignal
Transmembrane
   Molecular functionTransferase
   Technical termComplete proteome

Gene Ontology (GO)

   Cellular componentlipid particle

Inferred from direct assay. Source: FlyBase

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 449431Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
PRO_0000021956

Regions

Topological domain19 – 412394Lumenal Potential
Transmembrane413 – 43321 Potential
Topological domain434 – 44916Cytoplasmic Potential

Experimental info

Sequence conflict941R → A in CAA57525. Ref.1
Sequence conflict148 – 1492EH → DD in CAA57079. Ref.5
Sequence conflict175 – 1762AA → R in CAA57079. Ref.5
Sequence conflict225 – 2262LL → VV in CAA57079. Ref.5
Sequence conflict287 – 2893RLR → QVG in CAA57079. Ref.5
Sequence conflict2891R → V in CAA57525. Ref.1
Sequence conflict3041P → T in CAA57079. Ref.5
Sequence conflict329 – 3302RA → AR in CAA57525. Ref.1
Sequence conflict3351D → A in CAA57079. Ref.5

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Sequences

Sequence LengthMass (Da)Tools
Q24319-1 [UniParc].

Last modified July 5, 2005. Version 2.
Checksum: E4BC30AF376DCCD9

FASTA44949,981
        10         20         30         40         50         60 
MMWKALLIAV LAIAHCQAVL ETDANTLVLL DNLAIRETHS IFFKSLQDRG FKLTYKLADD 

        70         80         90        100        110        120 
SSLLLSKYGE YLYKNVIIFA PSVEEFGGDV SVERLAQFVD DGGNVLVAGS EKSGDALREF 

       130        140        150        160        170        180 
ASECGFELDE ENAAVIDHLH YDVSDAGEHT TILTSAKNLI QADTIVGKAN RQADAAPLLY 

       190        200        210        220        230        240 
RGTGLIADKE NPLVLKLLTA ESTAYSYNPE ASVSDYPHAV GRGTLLIAAL QARNNARVVF 

       250        260        270        280        290        300 
SGSLLFFSDE SFTTAVQYAQ SGVFHKLAGN RDVAESISKW VFGETGRLRV ASVQHHKEGE 

       310        320        330        340        350        360 
LLPPDQAYTI TDPVVYTIGI EELVQGEWRA FKASDIQLEF VRIDPFVRTY LKQTNTGAYQ 

       370        380        390        400        410        420 
AKFKIPDVYG VYQFKVDYNR VGYTHLYSTT QVSVRPLEHT QYERFIPSAF PYYTSAFSMM 

       430        440 
IGVFVFSFVF LHFKDEPVGR AAKEDKKSQ

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References

« Hide 'large scale' references
[1]"PCR-mediated cloning and sequencing of the DmOST50 gene, a WBP1/AvOST50/OST48 homologue, from Drosophila melanogaster."
Stagljar I., Te Heesen S., Aebi M.
Gene 158:209-212(1995) [PubMed: 7607543] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
[4]Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. expand/collapse author list , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[5]"Structure and expression of histone H3.3 genes in Drosophila melanogaster and Drosophila hydei."
Akhmanova A.S., Bindels P.S.T., Xu J., Miedema K., Kremer H., Hennig W.
Genome 38:586-600(1995) [PubMed: 7557364] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 119-449.

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Cross-references

Sequence databases

X81999 mRNA. Translation: CAA57525.1.
AE014298 Genomic DNA. Translation: AAF46453.1.
BT010050 mRNA. Translation: AAQ22519.1.
X81207 Genomic DNA. Translation: CAA57079.1.
PIRJC4132.
RefSeqNP_511096.2.
UniGeneDm.2930

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ24319.

Genome annotation databases

EnsemblCG9022. Drosophila melanogaster. [Contig view]
GeneID31849.
KEGGdme:Dmel_CG9022.
NMPDRfig|7227.3.peg.17103.

Organism-specific databases

FlyBaseFBgn0014868. Ost48.

Phylogenomic databases

HOGENOMQ24319.

Gene expression databases

ArrayExpressQ24319.
GermOnlineCG9022. Drosophila melanogaster.

Family and domain databases

InterProIPR005013. DDOST_48kDa.
[Graphical view]
PANTHERPTHR10830. DDOST_48kDa. 1 hit.
PfamPF03345. DDOST_48kD. 1 hit.
[Graphical view]
BLOCKSSearch...
ProtoNetSearch...

Other Resources

NextBio775646.

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Entry information

Entry nameOST48_DROME
AccessionPrimary (citable) accession number: Q24319
Secondary accession number(s): Q24355, Q9W382
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 5, 2005
Last modified: November 4, 2008
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents