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Reviewed, UniProtKB/Swiss-Prot Q24139 (COMM_DROME)

Last modified November 4, 2008. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Protein commissureless
Gene names
Name: comm
ORF Names: CG17943
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Controls axon guidance across the CNS midline by preventing the delivery of Robo to the growth cone.

Subunit structure

Interacts with Nedd4 and Robo.

Subcellular location

Cytoplasmic vesicle membrane; Single-pass membrane proteinPotential. Cell membrane; Single-pass membrane proteinPotential.

Domain

The cytoplasmic domain is required for function in axon guidance.

Post-translational modification

According to Ref.4, ubiquitinated by Nedd4; which promotes endocytosis of the comm/robo complex and comm proteasomal degradation. According to Ref.5, not ubiquitinated by Nedd4.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

RpS9Q9VT06-11EBI-118294,EBI-237039

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Protein commissureless
PRO_0000090013

Regions

Topological domain1 – 136136Extracellular Potential
Transmembrane137 – 15721 Potential
Topological domain158 – 370213Cytoplasmic Potential
Region108 – 13124Required for vesicular localization
Region220 – 2234Interaction with Nedd4
Region229 – 2324Interaction with Nedd4
Compositional bias12 – 154Poly-Thr
Compositional bias258 – 2625Poly-His
Compositional bias283 – 2864Poly-Ala
Compositional bias294 – 3018Poly-Ser
Compositional bias365 – 3684Poly-Val

Experimental info

Mutagenesis2211P → A: Abolishes interaction with Nedd4, ubiquitination and internalization; when associated with A-230
Mutagenesis2231Y → A: No effect on interaction with Nedd4
Mutagenesis2301P → A: Abolishes interaction with Nedd4, ubiquitination and internalization; when associated with A-221
Mutagenesis2321Y → A: No effect on interaction with Nedd4

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Sequences

Sequence LengthMass (Da)Tools
Q24139-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EB298EA415D4FBDA

FASTA37040,311
        10         20         30         40         50         60 
MISTTDYPTV ETTTTAEELY AEYISAPASS MSPAAIAEHL QQNQITFEIP SAHDLRHIDA 

        70         80         90        100        110        120 
LNSFNALLQR IGNAAVSYDP APPSGWSPDG SISTEQLSKS VVLDLADLRD RSEESGESSW 

       130        140        150        160        170        180 
WSQIFGDADM HVIINYLWIG VVSSLVILSL VFILFSCYFY RKFRTWKKCN KDIRAQIHAA 

       190        200        210        220        230        240 
SDSYSSHLVG CDASRLLLHQ QMQHPHHRSS EAGFYQIESP PCYTIATGLP SYDEALHHQP 

       250        260        270        280        290        300 
RHFAYGMKFV YPSLAAVHHH HHCISNWEKQ EPLNKLQKCK LSAAAAVEED KADSSSSTSA 

       310        320        330        340        350        360 
SASPSSSESS NLATATPAIC INMPSGRQDE EVDNSDSDSA IAVAVAVAQS LQPAAPADDD 

       370 
CASLVVVVAA

« Hide

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References

« Hide 'large scale' references
[1]"Commissureless controls growth cone guidance across the CNS midline in Drosophila and encodes a novel membrane protein."
Tear G., Harris R., Sutaria S., Kilomanski K., Goodman C.S., Seeger M.A.
Neuron 16:501-514(1996) [PubMed: 8785048] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
[4]"Drosophila Nedd4, a ubiquitin ligase, is recruited by Commissureless to control cell surface levels of the roundabout receptor."
Myat A., Henry P., McCabe V., Flintoft L., Rotin D., Tear G.
Neuron 35:447-459(2002) [PubMed: 12165468] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NEDD4 AND ROBO, UBIQUITINATION, MUTAGENESIS OF PRO-221; TYR-223; PRO-230 AND TYR-232, SUBCELLULAR LOCATION.
[5]"Comm function in commissural axon guidance: cell-autonomous sorting of Robo in vivo."
Keleman K., Ribeiro C., Dickson B.J.
Nat. Neurosci. 8:156-163(2005) [PubMed: 15657595] [Abstract]
Cited for: FUNCTION, LACK OF UBIQUITINATION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U35854 mRNA. Translation: AAC47019.1.
AE014296 Genomic DNA. Translation: AAF49584.1.
RefSeqNP_524095.1.
UniGeneDm.2546

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2EZ5NMR-P227-237[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:22799N.
IntActQ24139.

Genome annotation databases

EnsemblCG17943. Drosophila melanogaster. [Contig view]
GeneID39717.
KEGGdme:Dmel_CG17943.
NMPDRfig|7227.3.peg.9993.

Organism-specific databases

FlyBaseFBgn0010105. comm.

Phylogenomic databases

HOGENOMQ24139.

Enzyme and pathway databases

BioCycDMEL-XXX-02:DMEL-XXX-02-016810-MON.

Gene expression databases

ArrayExpressQ24139.
GermOnlineCG17943. Drosophila melanogaster.

Family and domain databases

BLOCKSSearch...
ProtoNetSearch...

Other Resources

NextBio815023.

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Entry information

Entry nameCOMM_DROME
AccessionPrimary (citable) accession number: Q24139
Secondary accession number(s): Q9VUT9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 4, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents