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Reviewed, UniProtKB/Swiss-Prot Q23985 (DTX_DROME)

Last modified November 4, 2008. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Protein deltex
Gene names
Name: dx
ORF Names: CG3929
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length738 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it may also act as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch. May function as an ubiquitin ligase protein in the Notch pathway.

Subunit structure

Homomultimer; the oligomerization is required for its function. Interacts with the ankyrin repeats of Notch.

Subcellular location

Cytoplasm.

Tissue specificity

Ubiquitous. Expressed at low levels throughout embryogenesis and in larvae.

Domain

The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases By similarity.

Sequence similarities

Belongs to the Deltex family.

Contains 1 RING-type zinc finger.

Contains 2 WWE domains.

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Ontologies

Keywords

   Biological processNotch signaling pathway
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   DomainRepeat
SH3-binding
Zinc-finger
   LigandMetal-binding
Zinc
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome

Gene Ontology (GO)

   Biological processWnt receptor signaling pathway Ref.8

Inferred from mutant phenotype. Source: UniProtKB

negative regulation of Notch signaling pathway Ref.7 Ref.9

Inferred from direct assay. Source: UniProtKB

positive regulation of Notch signaling pathway Ref.7 Ref.9

Inferred from direct assay. Source: UniProtKB

   Cellular componentcytoplasm Ref.1

Inferred from direct assay. Source: UniProtKB

   Molecular functionNotch binding Ref.9

Inferred from physical interaction. Source: UniProtKB

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 738738Protein deltex
PRO_0000219079

Regions

Domain35 – 11884WWE 1
Domain119 – 20183WWE 2
Zinc finger548 – 60356RING-type
Region1 – 304304Interaction with Notch
Motif480 – 4845SH3-binding Potential
Compositional bias251 – 30353Gln/His-rich (OPA repeat)
Compositional bias332 – 472141Ser-rich
Compositional bias489 – 51426Poly-Gln (OPA repeat)

Amino acid modifications

Modified residue4251Phosphoserine

Natural variations

Natural variant5111Q → L in strain: ZW141.
Natural variant5141Q → QQ in strain: ZW122.

Experimental info

Mutagenesis476 – 4849Missing: Dominant-negative mutant that inhibits Notch signaling
Mutagenesis5711H → A: Loss of function. Abolishes homomultimerization; when associated with A-574
Mutagenesis5741H → A: Loss of function. Abolishes homomultimerization; when associated with A-571
Sequence conflict107 – 1082AG → R in AAA18501. Ref.1
Sequence conflict1201F → L in AAA18501. Ref.1
Sequence conflict188 – 1892QL → HV in AAA18501. Ref.1
Sequence conflict2681H → L in AAA18501. Ref.1
Sequence conflict4441R → S in AAO39479. Ref.5
Sequence conflict4681L → V in AAA18501. Ref.1

Secondary structure

1 .................................... 738
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q23985-1 [UniParc].

Last modified August 15, 2003. Version 2.
Checksum: 7B1CF29E024D26AF

FASTA73882,186
        10         20         30         40         50         60 
MASSAGSAAS GSVVPGGGGS AASSCATMAL STAGSGGPPV NHAHAVCVWE FESRGKWLPY 

        70         80         90        100        110        120 
SPAVSQHLER AHAKKLTRVM LSDADPSLEQ YYVNVRTMTQ ESEAETAGSG LLTIGVRRMF 

       130        140        150        160        170        180 
YAPSSPAGKG TKWEWSGGSA DSNNDWRPYN MHVQCIIEDA WARGEQTLDL CNTHIGLPYT 

       190        200        210        220        230        240 
INFCNLTQLR QPSGPMRSIR RTQQAPYPLV KLTPQQANQL KSNSASVSSQ YNTLPKLGDT 

       250        260        270        280        290        300 
KSLHRVPMTR QQHPLPTSHQ VQQQQHQHQH QQQQQQQHHH QHQQQQHQQQ QQHQMQHHQI 

       310        320        330        340        350        360 
HHQTAPRKPP KKHSEISTTN LRQILNNLNI FSSSTKHQSN MSTAASASSS SSSASLHHAN 

       370        380        390        400        410        420 
HLSHAHFSHA KNMLTASMNS HHSRCSEGSL QSQRSSRMGS HRSRSRTRTS DTDTNSVKSH 

       430        440        450        460        470        480 
RRRPSVDTVS TYLSHESKES LRSRNFAISV NDLLDCSLGS DEVFVPSLPP SSLGERAPVP 

       490        500        510        520        530        540 
PPLPLHPRQQ QQQQQQQQQL QMQQQQQAQQ QQQQSIAGSI VGVDPASDMI SRFVKVVEPP 

       550        560        570        580        590        600 
LWPNAQPCPM CMEELVHSAQ NPAISLSRCQ HLMHLQCLNG MIIAQQNEMN KNLFIECPVC 

       610        620        630        640        650        660 
GIVYGEKVGN QPIGSMSWSI ISKNLPGHEG QNTIQIVYDI ASGLQTEEHP HPGRAFFAVG 

       670        680        690        700        710        720 
FPRICYLPDC PLGRKVLRFL KIAFDRRLLF SIGRSVTTGR EDVVIWNSVD HKTQFNMFPD 

       730 
PTYLQRTMQQ LVHLGVTD

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References

« Hide 'large scale' references
[1]"A member of the Notch group of interacting loci, deltex encodes a cytoplasmic basic protein."
Busseau I., Diederich R.J., Xu T., Artavanis-Tsakonas S.
Genetics 136:585-596(1994) [PubMed: 8150285] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: Oregon-R.
Tissue: Embryo.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
[4]Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[5]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-738.
Strain: Berkeley.
Tissue: Embryo.
[6]"Hitchhiking effects of recurrent beneficial amino acid substitutions in the Drosophila melanogaster genome."
Andolfatto P.
Genome Res. 17:1755-1762(2007) [PubMed: 17989248] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 326-567, VARIANTS LEU-511 AND GLN-514 INS.
Strain: ZW104, ZW109, ZW122, ZW123, ZW133, ZW136, ZW139, ZW140, ZW141, ZW142, ZW143 and ZW144.
[7]"Deltex acts as a positive regulator of Notch signaling through interactions with the Notch ankyrin repeats."
Matsuno K., Diederich R.J., Go M.J., Blaumueller C.M., Artavanis-Tsakonas S.
Development 121:2633-2644(1995) [PubMed: 7671825] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NOTCH.
[8]"Novel Notch alleles reveal a Deltex-dependent pathway repressing neural fate."
Ramain P., Khechumian K., Seugnet L., Arbogast N., Ackermann C., Heitzler P.
Curr. Biol. 11:1729-1738(2001) [PubMed: 11719214] [Abstract]
Cited for: FUNCTION.
[9]"Involvement of a proline-rich motif and RING-H2 finger of Deltex in the regulation of Notch signaling."
Matsuno K., Ito M., Hori K., Miyashita F., Suzuki S., Kishi N., Artavanis-Tsakonas S., Okano H.
Development 129:1049-1059(2002) [PubMed: 11861487] [Abstract]
Cited for: FUNCTION, HOMOMULTIMERIZATION, MUTAGENESIS OF 476-ARG--PRO-484; HIS-571 AND HIS-574.
[10]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-425, MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U09789 mRNA. Translation: AAA18501.1.
AE014298 Genomic DNA. Translation: AAF46170.1.
BT033059 mRNA. Translation: ACE82582.1.
BT003476 mRNA. Translation: AAO39479.1. Different initiation.
EU217842 Genomic DNA. Translation: ABW92761.1.
EU217843 Genomic DNA. Translation: ABW92762.1.
EU217844 Genomic DNA. Translation: ABW92763.1.
EU217845 Genomic DNA. Translation: ABW92764.1.
EU217846 Genomic DNA. Translation: ABW92765.1.
EU217847 Genomic DNA. Translation: ABW92766.1.
EU217848 Genomic DNA. Translation: ABW92767.1.
EU217849 Genomic DNA. Translation: ABW92768.1.
EU217850 Genomic DNA. Translation: ABW92769.1.
EU217851 Genomic DNA. Translation: ABW92770.1.
EU217852 Genomic DNA. Translation: ABW92771.1.
EU217853 Genomic DNA. Translation: ABW92772.1.
PIRS47857.
RefSeqNP_511064.2.
UniGeneDm.2626

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2A90X-ray2.15A26-261[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:18159N.
IntAct