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Reviewed, UniProtKB/Swiss-Prot P52656 (T2AG_DROME)

Last modified November 4, 2008. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Transcription initiation factor IIA subunit 2
Alternative name(s):
    General transcription factor IIA subunit 2
    Transcription initiation factor IIA gamma chain
      Short name=TFIIA-gamma
    TFIIA p14 subunit
      Short name=TFIIA-14
    dTFIIA-S
Gene names
Name: TfIIA-S
ORF Names: CG5163
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length106 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.

Subunit structure

TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p30), a beta (p20) and a gamma (p14) subunit.

Subcellular location

Nucleus.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the TFIIA subunit 2 family.

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Ontologies

Keywords

   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Technical termComplete proteome
Direct protein sequencing

Gene Ontology (GO)

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

TfIIA-LP526541EBI-123680,EBI-132413

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 106106Transcription initiation factor IIA subunit 2
PRO_0000194048

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Sequences

Sequence LengthMass (Da)Tools
P52656-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 978556B6AA8B31CA

FASTA10612,193
        10         20         30         40         50         60 
MSYQLYRNTT LGNTLQESLD ELIQYGQITP GLAFKVLLQF DKSINNALNQ RVKARVTFKA 

        70         80         90        100 
GKLNTYRFCD NVWTLMLNDV EFREVHEIVK VDKVKIVACD GKSGEF

« Hide

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References

« Hide 'large scale' references
[1]"Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation."
Yokomori K., Zeidler M.P., Chen J.-L., Verrijzer C.P., Mlodzik M., Tjian R.
Genes Dev. 8:2313-2323(1994) [PubMed: 7958898] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 42-59, SUBUNIT.
Strain: Oregon-R.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.

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Cross-references

Sequence databases

X83271 Genomic DNA. Translation: CAA58244.1.
AE014297 Genomic DNA. Translation: AAF56199.1.
PIRA55121. A54883.
RefSeqNP_524467.1.
UniGeneDm.20591

3D structure databases

HSSPHSSP built from PDB template 1YTF based on UniProtKB P32774.
SMRP52656. Positions 3-100.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:23236N.
IntActP52656.

Genome annotation databases

EnsemblCG5163. Drosophila melanogaster. [Contig view]
GeneID42822.
KEGGdme:Dmel_CG5163.
NMPDRfig|7227.3.peg.14305.

Organism-specific databases

FlyBaseFBgn0013347. TfIIA-S.

Phylogenomic databases

HOGENOMP52656.

Enzyme and pathway databases

BioCycDMEL-XXX-02:DMEL-XXX-02-013054-MON.

Gene expression databases

GermOnlineCG5163. Drosophila melanogaster.

Family and domain databases

InterProIPR009088. TFIIA_b-brl.
IPR003194. TFIIA_gsu.
IPR015871. TFIIA_gsu_C.
IPR015872. TFIIA_gsu_N.
[Graphical view]
Gene3DG3DSA:2.30.18.10. TFIIA_betabarrel. 1 hit.
G3DSA:1.10.287.190. TFIIA_gsu_N. 1 hit.
PANTHERPTHR10966. TFIIA_gamma. 1 hit.
PfamPF02751. TFIIA_gamma_C. 1 hit.
PF02268. TFIIA_gamma_N. 1 hit.
[Graphical view]
PIRSFPIRSF009415. Hum_TFIIA_gamma. 1 hit.
ProDomPD009224. TFIIA_gamma. 1 hit.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...
ProtoNetSearch...

Other Resources

NextBio830743.

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Entry information

Entry nameT2AG_DROME
AccessionPrimary (citable) accession number: P52656
Secondary accession number(s): Q9VCG7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 4, 2008
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents