Rho-GTPase-activating protein LRG1 - Saccharomyces cerevisiae (Baker's yeast)

Names and origin

Protein names

Recommended name:
Rho-GTPase-activating protein LRG1
Alternative name(s):
LIM-RhoGAP protein 1

Gene names

Name:	LRG1
Ordered Locus Names:	YDL240W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	1017 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Acts in signal transduction. Activates CDC42, RHO1 and RHO2. Negatively regulates 1,3-beta-glucan synthesis. May be responsible for the down-regulation of CDC42 during mating.
Subunit structure	Interacts with CDC42, RHO1 and RHO2.
Subcellular location	Cytoplasm. Bud. Bud neck. Note= Localized to the bud site during bud formation and at the bud neck during cytokinesis.
Developmental stage	Highly expressed during sporulation.
Miscellaneous	Present with 468 molecules/cell in log phase SD medium.
Sequence similarities	Contains 3 LIM zinc-binding domains. Contains 1 Rho-GAP domain.

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Ontologies

Keywords
Biological process	Sporulation
Cellular component	Cytoplasm
Domain	LIM domain Repeat
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fungal-type cell wall biogenesis Ref.4 Inferred from genetic interaction. Source: SGD small GTPase mediated signal transduction Ref.3 Inferred from physical interaction. Source: SGD
Cellular component	cellular bud neck Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD
Molecular function	Rho GTPase activator activity Ref.3 Inferred from direct assay. Source: SGD
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 1017

1017

Rho-GTPase-activating protein LRG1

Regions

Domain

28 – 88

61

LIM zinc-binding 1

Domain

98 – 148

51

LIM zinc-binding 2

Domain

155 – 184

30

LIM zinc-binding 3; truncated

Domain

419 – 474

56

LIM zinc-binding 4

Domain

730 – 953

224

Rho-GAP

Amino acid modifications

Modified residue

562

1

Phosphoserine

Experimental info

Sequence conflict

531

1

H → Q in CAA55210. Ref.1

Sequence conflict

766

1

R → S in CAA55210. Ref.1

Sequence conflict

791

1

N → T in CAA55210. Ref.1

Sequence conflict

821

1

L → Q in CAA55210. Ref.1

Sequence conflict

838

1

A → S in CAA55210. Ref.1

Sequence conflict

849

1

V → L in CAA55210. Ref.1

Sequence conflict

895

1

S → F in CAA55210. Ref.1

Sequence conflict

928

1

A → T in CAA55210. Ref.1

Sequence conflict

935

1

Y → S in CAA55210. Ref.1

Sequence conflict

977 – 1017

41

INHFI…GEINK → TILFPPLCKVKQSIIPNVT in CAA55210. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P35688-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: DDEB79B4BBEBBB09
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FASTA

1,017

116,660

        10         20         30         40         50         60 
MIQNSAGYRS LNTASPMTVQ VKNQKKICAR CNKLVIPDSQ RTKTTLKALG KYYHESCFTC 

        70         80         90        100        110        120 
QDCQKPLKPK YFPYQVDKTS ESILLCQYDY FRRHNLLCHV CDTPLRGLYY TAFGYRYDEE 

       130        140        150        160        170        180 
HFSCTICATP CGVKKCFMYG NQLYCKYHFL KYFSKRCKGC EFPISDQYIE FPKGEEIHCW 

       190        200        210        220        230        240 
HPECYGIHKY WHVNLAAETV GLQYLPKLEY NPNSGDKDIN PTAYELDKQM QAFNFILSKT 

       250        260        270        280        290        300 
WSVLYRFEEE AASCISDMFQ YLTSNDQLKG IESTGLLVLK IDCLFRGLDT LNLSTNKSMP 

       310        320        330        340        350        360 
VNSDQECIEN NAMAASKYSK FPKNLSTKIM IYLQLLRKLG TENKNETITI SSFMSVITGL 

       370        380        390        400        410        420 
AHFLKLLTRF GLYTALENNK LTHSVNPLLR FLREVEKNEL FENNPFQYIK TPVNATDSCA 

       430        440        450        460        470        480 
GCNKYIQEEC IQFYEHRWHI ACFTCSSCHK NINPRSLTDP TFNKEKKKIL CSHCSIDDPA 

       490        500        510        520        530        540 
SVPGFKFVTK LAQLIFLLKI ALVKSRTVML KSKASNKVGR NSLQSTMLKE HTYIRTLNDI 

       550        560        570        580        590        600 
KRLRSRRESV RVTHNKQQAR KSVILETAET DLNDPTKQGD SKNLVIQTDD PSSSQQVSTR 

       610        620        630        640        650        660 
ENVFSNTKTL TLDDISRIVA AEQARELRPN AFAHFKKLKE TDDETSNVVP KKSGVYYSEL 

       670        680        690        700        710        720 
STMELSMIRA ISLSLLAGKQ LISKTDPNYT SLVSMVFSNE KQVTGSFWNR MKIMMSMEPK 

       730        740        750        760        770        780 
KPITKTVFGA PLDVLCEKWG VDSDLGVGPV KIRIPIIIDE LISSLRQMDM SVEGIFRKNG 

       790        800        810        820        830        840 
NIRRLRELTA NIDSNPTEAP DFSKENAIQL SALLKKFIRE LPQPILSTDL YELWIKAAKI 

       850        860        870        880        890        900 
DLEDEKQRVI LLIYSLLPTY NRNLLEALLS FLHWTSSFSY IENEMGSKMD IHNLSTVITP 

       910        920        930        940        950        960 
NILYLRHKEI SNDNVPDEPE SGLVDSFAQN KGENYFLAIE IVDYLITHNE EMAMVPKFLM 

       970        980        990       1000       1010 
NLLKDVQLQK LDNYESINHF ISTVMQSKTI DYSECDIKTP VTVKDSTTTV IQGEINK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"LRG1 is expressed during sporulation in Saccharomyces cerevisiae and contains motifs similar to LIM and rho/racGAP domains." Mueller A., Xu G., Wells R., Hollenberg C.P., Piepersberg W. Nucleic Acids Res. 22:3151-3154(1994) [PubMed: 8065929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DBY874.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae." Roumanie O., Weinachter C., Larrieu I., Crouzet M., Doignon F. FEBS Lett. 506:149-156(2001) [PubMed: 11591390] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC42; RHO1 AND RHO2.
[4]	"Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3-beta-glucan synthesis." Watanabe D., Abe M., Ohya Y. Yeast 18:943-951(2001) [PubMed: 11447600] [Abstract] Cited for: FUNCTION, INTERACTION WITH RHO1, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 0:0-0(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, MASS SPECTROMETRY.

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Cross-references

Sequence databases
	X78453 Genomic DNA. Translation: CAA55210.1. Z74288 Genomic DNA. Translation: CAA98820.1.
PIR	S67804.
RefSeq	NP_010041.1.
3D structure databases
HSSP	HSSP built from PDB template 1RGP based on UniProtKB Q07960.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2588N.
Genome annotation databases
Ensembl	YDL240W. Saccharomyces cerevisiae. [Contig view]
GeneID	851358.
GenomeReviews	Gene locus YDL240W in contig Z71256_GR.
KEGG	sce:YDL240W.
NMPDR	fig\|4932.3.peg.774.
Organism-specific databases
CYGD	YDL240w.
SGD	S000002399. LRG1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P35688.
Gene expression databases
ArrayExpress	P35688.
GermOnline	YDL240W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000198. RhoGAP. IPR001781. Znf_LIM. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit. G3DSA:2.10.110.10. Znf_LIM. 2 hits.
Pfam	PF00412. LIM. 3 hits. PF00620. RhoGAP. 1 hit. [Graphical view]
ProDom	PD000094. LIM. 3 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00132. LIM. 3 hits. SM00324. RhoGAP. 1 hit. [Graphical view]
PROSITE	PS00478. LIM_DOMAIN_1. 2 hits. PS50023. LIM_DOMAIN_2. 3 hits. PS50238. RHOGAP. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
LinkHub	P35688.
ProtoNet	Search...

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Entry information

Entry name

LRG1_YEAST

Accession

Primary (citable) accession number: P35688
Secondary accession number(s): Q07735

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	November 1, 1997
Last modified:	July 22, 2008
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Recent format changes

Overview of recent format changes

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents