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Reviewed, UniProtKB/Swiss-Prot P23128 (DDX6_DROME)

Last modified July 22, 2008. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Putative ATP-dependent RNA helicase me31b
    EC=3.6.1.-
Alternative name(s):
    Maternal expression at 31B
Gene names
Name: me31B
ORF Names: CG4916
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length459 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Unwinds RNA in an ATP-dependent fashion Potential.

Developmental stage

Expressed both maternally and zygotically.

Sequence similarities

Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

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Ontologies

Keywords

   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionHelicase
Hydrolase
   PTMPhosphoprotein
   Technical termComplete proteome

Gene Ontology (GO)

   Molecular functionATP-dependent RNA helicase activity

Non-traceable author statement. Source: FlyBase

RNA binding

Inferred from direct assay. Source: FlyBase

protein binding

Inferred from physical interaction. Source: UniProtKB

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

Q9W1V71EBI-300281,EBI-168552
cupQ9VMA31EBI-300281,EBI-95398
mei-P26Q9W3781EBI-300281,EBI-187428

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: P23128-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: P23128-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.
Notes: No experimental confirmation available.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 459459Putative ATP-dependent RNA helicase me31b

Regions

Domain89 – 259171Helicase ATP-binding
Domain269 – 429161Helicase C-terminal
Nucleotide binding102 – 1098ATP By similarity
Motif58 – 8629Q motif
Motif207 – 2104DEAD box

Amino acid modifications

Modified residue81Phosphoserine
Modified residue291Phosphoserine
Modified residue4501Phosphoserine

Natural variations

Alternative sequence1 – 3131Missing in isoform B.

Experimental info

Sequence conflict39 – 457KLPPKDN → NCRQRTT in AAA28603. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified February 2, 2004. Version 3.
Checksum: B783E8185EF11E86

FASTA45951,945
        10         20         30         40         50         60 
MMTEKLNSGH TNLTSKGIIN DLQIAGNTSD DMGWKSKLKL PPKDNRFKTT DVTDTRGNEF 

        70         80         90        100        110        120 
EEFCLKRELL MGIFEKGWER PSPIQEAAIP IALSGKDVLA RAKNGTGKTG AYCIPVLEQI 

       130        140        150        160        170        180 
DPTKDYIQAL VMVPTRELAL QTSQICIELA KHLDIRVMVT TGGTILKDDI LRIYQKVQLI 

       190        200        210        220        230        240 
IATPGRILDL MDKKVADMSH CRILVLDEAD KLLSLDFQGM LDHVILKLPK DPQILLFSAT 

       250        260        270        280        290        300 
FPLTVKNFME KHLREPYEIN LMEELTLKGV TQYYAFVQER QKVHCLNTLF SKLQINQSII 

       310        320        330        340        350        360 
FCNSTQRVEL LAKKITELGY CCYYIHAKMA QAHRNRVFHD FRQGLCRNLV CSDLFTRGID 

       370        380        390        400        410        420 
VQAVNVVINF DFPRMAETYL HRIGRSGRFG HLGIAINLIT YEDRFDLHRI EKELGTEIKP 

       430        440        450 
IPKVIDPALY VANVGASVGD TCNNSDLNNS ANEEGNVSK

« Hide

Isoform B [UniParc].

Checksum: 34E61B3C97D11A2A
Show »

42848,644

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References

« Hide 'large scale' references
[1]"A second maternally expressed Drosophila gene encodes a putative RNA helicase of the 'DEAD box' family."
de Valoir T., Tucker M.A., Belikoff E.J., Camp L.A., Bolduc C., Beckingham K.
Proc. Natl. Acad. Sci. U.S.A. 88:2113-2117(1991) [PubMed: 1900936] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), DEVELOPMENTAL STAGE.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Berkeley.
Tissue: Embryo.
[5]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8; SER-29 AND SER-450, MASS SPECTROMETRY.
Tissue: Embryo.

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Cross-references

Sequence databases

M59926 mRNA. Translation: AAA28603.1.
AE014134 Genomic DNA. Translation: AAF52881.2.
AE014134 Genomic DNA. Translation: AAN10728.1.
AY051663 mRNA. Translation: AAK93087.1.
PIRA39157.
RefSeqNP_523533.2.
NP_723539.1.
UniGeneDm.2770

3D structure databases

HSSPHSSP built from PDB template 1QDE based on UniProtKB P10081.
SMRP23128. Positions 58-432.
ModBaseSearch...

Protein-protein interaction databases

IntActP23128.

Genome annotation databases

EnsemblCG4916. Drosophila melanogaster. [Contig view]
GeneID34364.
KEGGdme:Dmel_CG4916.

Organism-specific databases

FlyBaseFBgn0004419. me31B.

Phylogenomic databases

HOGENOMP23128.

Enzyme and pathway databases

BioCycDMEL-XXX-02:DMEL-XXX-02-008302-MON.

Gene expression databases

ArrayExpressP23128.
GermOnlineCG4916. Drosophila melanogaster.

Family and domain databases

InterProIPR014001. DEAD-like_N.
IPR001650. DNA/RNA_helicase_C.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014021. Helicase_SF1/SF2_ATP-bd.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProDomP23128.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameDDX6_DROME
AccessionPrimary (citable) accession number: P23128
Secondary accession number(s): Q8IPC9, Q961D2, Q9VL17
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: February 2, 2004
Last modified: July 22, 2008
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents