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Reviewed, UniProtKB/Swiss-Prot P20815 (CP3A5_HUMAN)

Last modified July 22, 2008. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 3A5
    EC=1.14.14.1
Alternative name(s):
    CYPIIIA5
    P450-PCN3
    HLp2
Gene names
Name: CYP3A5
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityPolymorphism
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase

Gene Ontology (GO)

   Biological processsteroid metabolic process Ref.1

Traceable author statement. Source: ProtInc

   Cellular componentmicrosome Ref.1

Traceable author statement. Source: ProtInc

   Molecular functionmonooxygenase activity Ref.1

Inferred from sequence or structural similarity. Source: UniProtKB

oxygen binding Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 502502Cytochrome P450 3A5

Sites

Metal binding4411Iron (heme axial ligand)

Natural variations

Natural variant281R → C in allele CYP3A5*8.
Natural variant301H → Y
Natural variant2001Q → R in allele CYP3A5*4.
Natural variant2771D → E
Natural variant3371A → T in allele CYP3A5*9.
Natural variant3711I → V
Natural variant3981T → N in allele CYP3A5*2. dbSNP rs28365083.
Natural variant4461F → S
Natural variant4881I → T

Experimental info

Sequence conflict3051A → P Ref.2
Sequence conflict3181L → F Ref.2
Sequence conflict3241H → D Ref.2
Sequence conflict3771C → G Ref.2

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Sequences

Sequence LengthMass (Da)Tools
P20815-1 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: D5A2302E2633E717

FASTA50257,109
        10         20         30         40         50         60 
MDLIPNLAVE TWLLLAVSLV LLYLYGTRTH GLFKRLGIPG PTPLPLLGNV LSYRQGLWKF 

        70         80         90        100        110        120 
DTECYKKYGK MWGTYEGQLP VLAITDPDVI RTVLVKECYS VFTNRRSLGP VGFMKSAISL 

       130        140        150        160        170        180 
AEDEEWKRIR SLLSPTFTSG KLKEMFPIIA QYGDVLVRNL RREAEKGKPV TLKDIFGAYS 

       190        200        210        220        230        240 
MDVITGTSFG VNIDSLNNPQ DPFVESTKKF LKFGFLDPLF LSIILFPFLT PVFEALNVSL 

       250        260        270        280        290        300 
FPKDTINFLS KSVNRMKKSR LNDKQKHRLD FLQLMIDSQN SKETESHKAL SDLELAAQSI 

       310        320        330        340        350        360 
IFIFAGYETT SSVLSFTLYE LATHPDVQQK LQKEIDAVLP NKAPPTYDAV VQMEYLDMVV 

       370        380        390        400        410        420 
NETLRLFPVA IRLERTCKKD VEINGVFIPK GSMVVIPTYA LHHDPKYWTE PEEFRPERFS 

       430        440        450        460        470        480 
KKKDSIDPYI YTPFGTGPRN CIGMRFALMN MKLALIRVLQ NFSFKPCKET QIPLKLDTQG 

       490        500 
LLQPEKPIVL KVDSRDGTLS GE

« Hide

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References

« Hide 'large scale' references
[1]"Cytochrome P-450 hPCN3, a novel cytochrome P-450 IIIA gene product that is differentially expressed in adult human liver. cDNA and deduced amino acid sequence and distinct specificities of cDNA-expressed hPCN1 and hPCN3 for the metabolism of steroid hormones and cyclosporine."
Aoyama T., Yamano S., Waxman D.J., Lapenson D.P., Meyer U.A., Fischer V., Tyndale R., Inaba T., Kalow W., Gelboin H.V., Gonzalez F.J.
J. Biol. Chem. 264:10388-10395(1989) [PubMed: 2732228] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Characterization of a cDNA encoding a new member of the glucocorticoid-responsive cytochromes P450 in human liver."
Schuetz J.D., Molowa D.T., Guzelian P.S.
Arch. Biochem. Biophys. 274:355-365(1989) [PubMed: 2802615] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Tissue: Liver.
[3]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed: 12853948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[5]"Genomic organization of the human CYP3A locus: identification of a new, inducible CYP3A gene."
Gellner K., Eiselt R., Hustert E., Arnold H., Koch I., Haberl M., Deglmann C.J., Burk O., Buntefuss D., Escher S., Bishop C., Koebe H.-G., Brinkmann U., Klenk H.-P., Kleine K., Meyer U.A., Wojnowski L.
Pharmacogenetics 11:111-121(2001) [PubMed: 11266076] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-106.
[6]"Identification of a novel dexamethasone responsive enhancer in the human CYP3A5 gene and its activation in human and rat liver cells."
Schuetz J.D., Schuetz E.G., Thottassery J.V., Guzelian P.S., Strom S., Sun D.
Mol. Pharmacol. 49:63-72(1996) [PubMed: 8569713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
[7]"Sequence of the 5'-flanking region of CYP3A5: comparative analysis with CYP3A4 and CYP3A7."
Jounaidi Y., Guzelian P.S., Maurel P., Vilarem M.J.
Biochem. Biophys. Res. Commun. 205:1741-1747(1994) [PubMed: 7811260] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
[8]"Detection of CYP3A5 allelic variant: a candidate for the polymorphic expression of the protein?"
Jounaidi Y., Hyrailles V., Gervot L., Maurel P.
Biochem. Biophys. Res. Commun. 221:466-470(1996) [PubMed: 8619878] [Abstract]
Cited for: VARIANT CYP3A5*2 ASN-398.
[9]"Genetic polymorphism of cytochrome P450 3A5 in Chinese."
Chou F.C., Tzeng S.J., Huang J.D.
Drug Metab. Dispos. 29:1205-1209(2001) [PubMed: 11502729] [Abstract]
Cited for: VARIANT CYP3A5*4 ARG-200.
[10]"Genetic findings and functional studies of human CYP3A5 single nucleotide polymorphisms in different ethnic groups."
Lee S.J., Usmani K.A., Chanas B., Ghanayem B., Xi T., Hodgson E., Mohrenweiser H.W., Goldstein J.A.
Pharmacogenetics 13:461-472(2003) [PubMed: 12893984] [Abstract]
Cited for: VARIANTS CYS-28; THR-337 AND SER-446.
[11]"Genetic variation in eleven phase I drug metabolism genes in an ethnically diverse population."
Solus J.F., Arietta B.J., Harris J.R., Sexton D.P., Steward J.Q., McMunn C., Ihrie P., Mehall J.M., Edwards T.L., Dawson E.P.
Pharmacogenomics 5:895-931(2004) [PubMed: 15469410] [Abstract]
Cited for: VARIANTS TYR-30; GLU-277; THR-337 AND ASN-398.

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Cross-references

Sequence databases

J04813 mRNA. Translation: AAA02993.1.
AC005020 Genomic DNA. Translation: AAS02016.1.
BC033862 mRNA. Translation: AAH33862.1.
AF280107 Genomic DNA. Translation: AAG32288.1.
L35912 Genomic DNA. Translation: AAB00083.1.
S74699 Genomic DNA. Translation: AAD14157.1.
S74700 Genomic DNA. Translation: AAD14158.1.
PIRA34101.
A60558.
RefSeqNP_000768.1.
UniGeneHs.695915

3D structure databases

HSSPHSSP built from PDB template 1JPZ based on UniProtKB P14779.
SMRP20815. Positions 29-498.
ModBaseSearch...

PTM databases

PhosphoSiteP20815.

Genome annotation databases

EnsemblENSG00000106258. Homo sapiens. [Contig view]
GeneID1577.
KEGGhsa:1577.

Organism-specific databases

H-InvDBHIX0006899.
HIX0034159.
HGNCHGNC:2638. CYP3A5.
MIM605325. gene.
PharmGKBPA131.
GenAtlasSearch...
GeneCardsSearch...
GeneLynxSearch...

Phylogenomic databases

HOGENOMP20815.
HOVERGENP20815.

Gene expression databases

ArrayExpressP20815.
CleanExHS_CYP3A5.
GermOnlineENSG00000106258. Homo sapiens.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR008072. Cyt_P450_E_CYP3A.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01689. EP450IICYP3A.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomP20815.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

DrugBankDB00802. Alfentanil.
DB00758. Clopidogrel.
DB00091. Cyclosporine.
DB00694. Daunorubicin.
DB00224. Indinavir.
DB00762. Irinotecan.
DB01026. Ketoconazole.
DB01259. Lapatinib.
DB00532. Mephenytoin.
DB00683. Midazolam.
DB00834. Mifepristone.
DB00252. Phenytoin.
DB00468. Quinine.
DB01232. Saquinavir.
DB00864. Tacrolimus.
DB01361. Troleandomycin.
DB00661. Verapamil.
DB00541. Vincristine.
SOURCESearch...
ProtoNetSearch...

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Entry information

Entry nameCP3A5_HUMAN
AccessionPrimary (citable) accession number: P20815
Secondary accession number(s): Q53WY8, Q75MV0, Q9HB56
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 22, 2008
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents