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Reviewed, UniProtKB/Swiss-Prot P18125 (CP7A1_RAT)

Last modified September 2, 2008. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 7A1
    EC=1.14.13.17
Alternative name(s):
    CYPVII
    Cholesterol 7-alpha-monooxygenase
    Cholesterol 7-alpha-hydroxylase
Gene names
Name: Cyp7a1
Synonyms: Cyp7
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

Cholesterol + NADPH + O(2) = 7-alpha-hydroxycholesterol + NADP(+) + H(2)O.

Cofactor

Heme group By similarity.

Enzyme regulation

Inhibition of activity by bile acid feedback.

Pathway

Lipid metabolism; bile acid biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Liver.

Induction

By cholestyramine and cholesterol.

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Biological processCholesterol metabolism
Lipid metabolism
Steroid metabolism
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Metal-binding
NADP
   Molecular functionMonooxygenase
Oxidoreductase

Gene Ontology (GO)

   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 503503Cytochrome P450 7A1

Sites

Metal binding4441Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict3711T → S in AAA41042. Ref.5

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Sequences

Sequence LengthMass (Da)Tools
P18125-1 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: EA825AA6E74BF5F6

FASTA50356,883
        10         20         30         40         50         60 
MMTISLIWGI AVLVSCCIWF IVGIRRRKAG EPPLENGLIP YLGCALKFGS NPLEFLRANQ 

        70         80         90        100        110        120 
RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF HYTTSAKAFG HRSIDPNDGN 

       130        140        150        160        170        180 
TTENINNTFT KTLQGDALCS LSEAMMQNLQ SVMRPPGLPK SKSNAWVTEG MYAFCYRVMF 

       190        200        210        220        230        240 
EAGYLTLFGR DISKTDTQKA LILNNLDNFK QFDQVFPALV AGLPIHLFKT AHKAREKLAE 

       250        260        270        280        290        300 
GLKHKNLCVR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL AILWASQANT IPATFWSLFQ 

       310        320        330        340        350        360 
MIRSPEAMKA ASEEVSGALQ SAGQELSSGG SAIYLDQVQL NDLPVLDSII KEALRLSSAS 

       370        380        390        400        410        420 
LNIRTAKEDF TLHLEDGSYN IRKDDMIALY PQLMHLDPEI YPDPLTFKYD RYLDESGKAK 

       430        440        450        460        470        480 
TTFYSNGNKL KCFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSCFELEFV ESQVKCPPLD 

       490        500 
QSRAGLGILP PLHDIEFKYK LKH

« Hide

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References

[1]"Regulation of cholesterol 7 alpha-hydroxylase in the liver. Cloning, sequencing, and regulation of cholesterol 7 alpha-hydroxylase mRNA."
Li Y.C., Wang D.P., Chiang J.Y.L.
J. Biol. Chem. 265:12012-12019(1990) [PubMed: 1694852] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"Cloning and regulation of cholesterol 7 alpha-hydroxylase, the rate-limiting enzyme in bile acid biosynthesis."
Jelinek D.F., Andersson S., Slaughter C.A., Russell D.W.
J. Biol. Chem. 265:8190-8197(1990) [PubMed: 2335522] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
[3]"Molecular cloning of cDNA for cholesterol 7 alpha-hydroxylase from rat liver microsomes. Nucleotide sequence and expression."
Noshiro M., Nishimoto M., Morohashi K., Okuda K.
FEBS Lett. 257:97-100(1989) [PubMed: 2806567] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Rat liver cholesterol 7 alpha-hydroxylase. Pretranslational regulation for circadian rhythm."
Noshiro M., Nishimoto M., Okuda K.
J. Biol. Chem. 265:10036-10041(1990) [PubMed: 1693613] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[5]"Structural analysis of the gene encoding rat cholesterol alpha-hydroxylase, the key enzyme for bile acid biosynthesis."
Nishimoto M., Gotoh O., Okuda K., Noshiro M.
J. Biol. Chem. 266:6467-6471(1991) [PubMed: 2007596] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"Identification and characterization of a putative bile acid-responsive element in cholesterol 7 alpha-hydroxylase gene promoter."
Chiang J.Y., Stroup D.
J. Biol. Chem. 269:17502-17507(1994) [PubMed: 8021257] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-325.
Strain: Sprague-Dawley.
[7]"Structure of the rat gene encoding cholesterol 7 alpha-hydroxylase."
Jelinek D.F., Russell D.W.
Biochemistry 29:7781-7785(1990) [PubMed: 2261433] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
Strain: Sprague-Dawley.
[8]"Cloning and 5'-flanking sequence of a rat cholesterol 7 alpha-hydroxylase gene."
Chiang J.Y., Yang T.P., Wang D.P.
Biochim. Biophys. Acta 1132:337-339(1992) [PubMed: 1420318] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.

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Cross-references

Sequence databases

J05509 mRNA. Translation: AAA40839.1.
J02926 Genomic DNA. Translation: AAA40923.1.
J05430 mRNA. Translation: AAA41041.1.
J05460 mRNA. Translation: AAA03649.1.
M59189 expand/collapse EMBL AC list , M59184, M59185, M59186, M59187, M59188 Genomic DNA. Translation: AAA41042.1.
U01962 Genomic DNA. Translation: AAA21144.2.
X17595 mRNA. Translation: CAB57878.1.
Z14108 Genomic DNA. Translation: CAA78481.1.
PIRA35376.
RefSeqNP_037074.1.
UniGeneRn.10737

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblENSRNOG00000009488. Rattus norvegicus. [Contig view]
GeneID25428.
KEGGrno:25428.

Organism-specific databases

RGD2482. Cyp7a1.

Phylogenomic databases

HOVERGENP18125.

Gene expression databases

ArrayExpressP18125.
GermOnlineENSRNOG00000009488. Rattus norvegicus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00465. EP450IV.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomP18125.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameCP7A1_RAT
AccessionPrimary (citable) accession number: P18125
Secondary accession number(s): P51543
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: September 2, 2008
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents