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Reviewed, UniProtKB/Swiss-Prot P15393 (C11B1_RAT)

Last modified September 2, 2008. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 11B1, mitochondrial
    EC=1.14.15.4
Alternative name(s):
    CYPXIB1
    P450C11
    Steroid 11-beta-hydroxylase
    P450(11 beta)-DS
Gene names
Name: Cyp11b1
Synonyms: Cyp11b-1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Forms corticosterone from 11-deoxycorticosterone.

Catalytic activity

A steroid + reduced adrenal ferredoxin + O(2) = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H(2)O.

Cofactor

Heme group By similarity.

Subcellular location

Mitochondrion membrane.

Tissue specificity

Adrenal zona fasciculata/reticularis.

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Biological processSteroidogenesis
   Cellular componentMembrane
Mitochondrion
   DomainTransit peptide
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Cellular componentmitochondrial membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Transit peptide1 – 2424Mitochondrion
Chain25 – 499475Cytochrome P450 11B1, mitochondrial

Sites

Metal binding4461Iron (heme axial ligand) By similarity

Natural variations

Natural variant1271R → C in strain: Dahl salt-resistant.
Natural variant3511V → A in strain: Dahl salt-resistant.
Natural variant3811V → L in strain: Dahl salt-resistant.
Natural variant3841I → L in strain: Dahl salt-resistant.
Natural variant4431V → M in strain: Dahl salt-resistant.

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Sequences

Sequence LengthMass (Da)Tools
P15393-1 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 19C041F96A673C7E

FASTA49957,459
        10         20         30         40         50         60 
MALRVTADVW LARPWQCLHR TRALGTTAKV APKTLKPFEA IPQYSRNKWL KMIQILREQG 

        70         80         90        100        110        120 
QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL HQVESILPHR MPLEPWVAHR 

       130        140        150        160        170        180 
ELRGLRRGVF LLNGADWRFN RLQLNPNMLS PKAIQSFVPF VDVVARDFVE NLKKRMLENV 

       190        200        210        220        230        240 
HGSMSINIQS NMFNYTMEAS HFVISGERLG LTGHDLKPES VTFTHALHSM FKSTTQLMFL 

       250        260        270        280        290        300 
PKSLTRWTST RVWKEHFDSW DIISEYVTKC IKNVYRELAE GRQQSWSVIS EMVAQSTLSM 

       310        320        330        340        350        360 
DAIHANSMEL IAGSVDTTAI SLVMTLFELA RNPDVQQALR QESLAAEASI VANPQKAMSD 

       370        380        390        400        410        420 
LPLLRAALKE TLRLYPVGSF VERIVHSDLV LQNYHVPAGT FVIIYLYSMG RNPAVFPRPE 

       430        440        450        460        470        480 
RYMPQRWLER KRSFQHLAFG FGVRQCLGRR LAEVEMLLLL HHMLKTFQVE TLRQEDMQMV 

       490 
FRFLLMPSSS PFLTFRPVS

« Hide

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References

[1]"Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P-450(11)beta."
Nonaka Y., Matsukawa N., Morohashi K., Omura T., Ogihara T., Teraoka H., Okamoto M.
FEBS Lett. 255:21-26(1989) [PubMed: 2551730] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
[2]"Dahl's salt-resistant normotensive rat has mutations in cytochrome P450(11 beta), but the salt-sensitive hypertensive rat does not."
Matsukawa N., Nonaka Y., Higaki J., Nagano M., Mikami H., Ogihara T., Okamoto M.
J. Biol. Chem. 268:9117-9121(1993) [PubMed: 8473350] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Dahl salt-resistant.
Tissue: Adrenal gland.
[3]"Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses."
Mukai K., Imai M., Shimada H., Ishimura Y.
J. Biol. Chem. 268:9130-9137(1993) [PubMed: 8473352] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
Tissue: Testis.
[4]"Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene."
Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T.
J. Biochem. 113:144-152(1993) [PubMed: 8468320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[5]"Cytochrome P450(11 beta): structure-function relationship of the enzyme and its involvement in blood pressure regulation."
Okamoto M., Nonaka Y., Ohta M., Takemori H., Halder S.K., Zhi-Nong W., Sun T., Hatano O., Takakusa A., Murakami T.
J. Steroid Biochem. Mol. Biol. 53:89-94(1995) [PubMed: 7626522] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Dahl salt-resistant.
[6]"Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex."
Ogishima T., Mitani F., Ishimura Y.
J. Biol. Chem. 264:10935-10938(1989) [PubMed: 2738055] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-44.
Tissue: Adrenal cortex.

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Cross-references

Sequence databases

D11354 mRNA. Translation: BAA01957.1.
D14091 Genomic DNA. Translation: BAA03171.1.
X15431 mRNA. Translation: CAA33472.1.
D10107 mRNA. Translation: BAA00988.1.
PIRA46039.
RefSeqNP_036669.2.
UniGeneRn.198235

3D structure databases

HSSPHSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBaseSearch...

Genome annotation databases

EnsemblENSRNOG00000032450. Rattus norvegicus. [Contig view]
GeneID500892.
KEGGrno:500892.

Organism-specific databases

RGD2453. Cyp11b1.

Phylogenomic databases

HOVERGENP15393.

Gene expression databases

ArrayExpressP15393.
GermOnlineENSRNOG00000032450. Rattus norvegicus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR002399. Cyt_P450_mit.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00408. MITP450.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomP15393.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameC11B1_RAT
AccessionPrimary (citable) accession number: P15393
Secondary accession number(s): Q64655
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 2, 2008
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents