Reviewed,
UniProtKB/Swiss-Prot P15392 (CP2A4_MOUSE)
Last modified
July 22, 2008.
Version 69.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Cytochrome P450 2A4 EC=1.14.14.1 Alternative name(s): CYPIIA4 Testosterone 15-alpha-hydroxylase P450-15-alpha P450-IIA3.1 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 494 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Highly active in the 15-alpha-hydroxylation of testosterone. Also active in the 15-alpha-hydroxylation of progesterone and androstenedione. Little or no activity on corticosterone, pregnenolone, dehydroepiandrosterone, estradiol or estriol. |
| Catalytic activity | RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O. |
| Cofactor | Heme group By similarity. |
| Subcellular location | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| Tissue specificity | Kidney and lung. Expressed in liver, with a strong circadian rhythmicity. Circadian expression is regulated by DBP. |
| Miscellaneous | There are only 11 differences between the sequence of testosterone 15-alpha-hydroxylase and that of coumarin 7-hydroxylase. By site-directed mutagenesis it has been shown that modification of position 209 is sufficient to convert the specificity of the two forms of the enzyme. |
| Sequence similarities | Belongs to the cytochrome P450 family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane Microsome |
| Ligand | Heme Iron Metal-binding |
| Molecular function | Monooxygenase Oxidoreductase |
Gene Ontology (GO) | |
| Cellular component | microsome Inferred from direct assay. Source: MGI |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| [1] | "The structure and characterization of type I P-450(15) alpha gene as major steroid 15 alpha-hydroxylase and its comparison with type II P-450(15) alpha gene." Lindberg R., Burkhart B., Ichikawa T., Negishi M. J. Biol. Chem. 264:6465-6471(1989) [PubMed: 2703500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver. |
| [2] | "Reciprocal regulation of sex-dependent expression of testosterone 15 alpha-hydroxylase (P-450(15 alpha)) in liver and kidney of male mice by androgen. Evidence for a single gene." Squires E.J., Negishi M. J. Biol. Chem. 263:4166-4171(1988) [PubMed: 3346244] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue." Lindberg R., Negishi M. Nature 339:632-634(1989) [PubMed: 2733794] [Abstract] Cited for: MUTAGENESIS. |
| [4] | "Circadian expression of the steroid 15 alpha-hydroxylase (Cyp2a4) and coumarin 7-hydroxylase (Cyp2a5) genes in mouse liver is regulated by the PAR leucine zipper transcription factor DBP." Lavery D.J., Lopez-Molina L., Margueron R., Fleury-Olela F., Conquet F., Schibler U., Bonfils C. Mol. Cell. Biol. 19:6488-6499(1999) [PubMed: 10490589] [Abstract] Cited for: TISSUE SPECIFICITY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
M26208  M26207 Genomic DNA. Translation: AAA37797.1. J03549 mRNA. Translation: AAA40426.1. M19319 mRNA. Translation: AAA40429.1. | |
| PIR | A33531. |
| UniGene | Mm.154643 Mm.389848 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1DT6 based on UniProtKB P00179. |
| SMR | P15392. Positions 31-494. |
| ModBase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUSG00000074254. Mus musculus. [Contig view] |
Organism-specific databases | |
| MGI | MGI:88596. Cyp2a4. |
Phylogenomic databases | |
| HOGENOM | P15392. |
| HOVERGEN | P15392. |
Gene expression databases | |
| GermOnline | ENSMUSG00000074254. Mus musculus. |
Family and domain databases | |
| InterPro | IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. IPR008067. Cyt_P450_E_grp-I_CYP2A-like. [Graphical view] |
| Gene3D | G3DSA:1.10.630.10. Cyt_P450. 1 hit. |
| PANTHER | PTHR19383. Cyt_P450. 1 hit. |
| Pfam | PF00067. p450. 1 hit. [Graphical view] |
| PRINTS | PR00463. EP450I. PR01684. EP450ICYP2A. PR00385. P450. |
| PROSITE | PS00086. CYTOCHROME_P450. 1 hit. [Graphical view] |
| ProDom | P15392. [Graphical view] [Entries sharing at least one domain] |
| BLOCKS | Search... |
Other Resources | |
| SOURCE | Search... |
| ProtoNet | Search... |
Entry information
| Entry name | CP2A4_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P15392 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |
