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Reviewed, UniProtKB/Swiss-Prot P12791 (CP2BA_MOUSE)

Last modified September 2, 2008. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 2B10
    EC=1.14.14.1
Alternative name(s):
    CYPIIB10
    Testosterone 16-alpha hydroxylase
    P450-16-alpha
    Clone PF3/46
    Cytochrome P450 2B20
    CYPIIB20
    P24
Gene names
Name: Cyp2b10
Synonyms: Cyp2b-10, Cyp2b20
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

Up-regulated by cannabidiol.

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityAlternative splicing
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   PTMPhosphoprotein
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P12791-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P12791-2)

The sequence of this isoform differs from the canonical sequence as follows:
     433-441: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 500500Cytochrome P450 2B10

Sites

Metal binding4451Iron (heme axial ligand)

Amino acid modifications

Modified residue1281Phosphoserine; by PKA By similarity

Natural variations

Alternative sequence433 – 4419Missing in isoform 2.

Experimental info

Sequence conflict551L → F in CAA68051. Ref.2
Sequence conflict1781V → I in CAA68051. Ref.2
Sequence conflict251 – 2533RHK → KHR in CAA68051. Ref.2
Sequence conflict280 – 2812NA → HT in CAA68051. Ref.2
Sequence conflict2981V → A in CAA68051. Ref.2
Sequence conflict3081H → R in CAA68051. Ref.2
Sequence conflict3211T → A in CAA68051. Ref.2
Sequence conflict3491S → T in CAA68051. Ref.2
Sequence conflict3761L → M in CAA68051. Ref.2
Sequence conflict4121Q → H in CAA68051. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: F660A00D8D0FBA94

FASTA50056,744
        10         20         30         40         50         60 
MEPSVLLLLA LLVGFLLLLA RGHPKSRGNF PPGPRPLPLL GNLLQMDRGG LLKSLIQLRE 

        70         80         90        100        110        120 
KYGDVFTVHL GPRPVVMLCG TDTIREALVG QAEAFSGRGT VAVVEPTFKE YGVIFANGER 

       130        140        150        160        170        180 
WKTLRRFSLA TMRDFGMGKR SVEERIQEEA QCLVEELRKS QGAPLDPTFL FQCITANVIC 

       190        200        210        220        230        240 
SIVFGERFEY TDRQFLRLLE LFYQTFSLIS SFSSQMFELF SGFLKYFPGA HRQISKNLQE 

       250        260        270        280        290        300 
LLDYIGHSVE RHKATLDPSV PRDFIDIYLL RMEKEKSNQN AEFHHQNLMM SVLSLFFVGT 

       310        320        330        340        350        360 
ETSSTTLHYG FLLMLKYPHV TEKVQKEIDQ VIGSHRLPTL DDRTKMPYSD AVIHEIQRFS 

       370        380        390        400        410        420 
DLIPIGVPHR VTKDTLFRGY LLPKNTEVYP ILSSALHDPQ YFEQPDSFNP DQFLDANGAL 

       430        440        450        460        470        480 
KKSEAFLPFS TGQIFDQKSV GKRICLGESI ARSELFLFFT SILQNFSVAS HVAPKDIDLT 

       490        500 
PKESGIGKIP PTYQICFLAR

« Hide

Isoform 2 [UniParc].

Checksum: B4C7AAE1435FC7C9
Show »

49155,740

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References

[1]"Rip locus: regulation of female-specific isozyme (I-P-450(16 alpha) of testosterone 16 alpha-hydroxylase in mouse liver, chromosome localization, and cloning of P-450 cDNA."
Noshiro M., Lakso M., Kawajiri K., Negishi M.
Biochemistry 27:6434-6443(1988) [PubMed: 3219345] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Isolation of a new mouse cDNA clone: hybrid form of cytochrome P450 2b10 and NADPH-cytochrome P450 oxidoreductase."
Damon M., Fautrel A., Marc N., Guillouzo A., Corcos L.
Biochem. Biophys. Res. Commun. 226:900-905(1996) [PubMed: 8831708] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-452 (ISOFORM 2).
Strain: BALB/c.
Tissue: Liver.
[3]"Purification and characterization of a mouse liver cytochrome P-450 induced by cannabidiol."
Bornheim L.M., Correia M.A.
Mol. Pharmacol. 36:377-383(1989) [PubMed: 2779523] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-15, INDUCTION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M21856 mRNA. Translation: AAA40425.1.
X99715 mRNA. Translation: CAA68051.1. Sequence problems.
PIRB31047.
UniGeneMm.218749

3D structure databases

HSSPHSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBaseSearch...

Genome annotation databases

EnsemblENSMUSG00000030483. Mus musculus. [Contig view]

Organism-specific databases

MGIMGI:88598. Cyp2b10.

Phylogenomic databases

HOGENOMP12791.
HOVERGENP12791.

Gene expression databases

ArrayExpressP12791.
GermOnlineENSMUSG00000030483. Mus musculus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. False negative.
[Graphical view]
ProDomP12791.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

SOURCESearch...
ProtoNetSearch...

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Entry information

Entry nameCP2BA_MOUSE
AccessionPrimary (citable) accession number: P12791
Secondary accession number(s): Q62397
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: September 2, 2008
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents