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Reviewed, UniProtKB/Swiss-Prot P11711 (CP2A1_RAT)

Last modified September 2, 2008. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 2A1
    EC=1.14.14.1
Alternative name(s):
    CYPIIA1
    Steroid hormones 7-alpha-hydroxylase
    Testosterone 7-alpha-hydroxylase
    P450-UT-F
Gene names
Name: Cyp2a1
Synonyms: Cyp2a-1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Highly active in the 7-alpha-hydroxylation of testosterone, progesterone and androstenedione.

Catalytic activity

RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Liver and testis.

Induction

By 3-methylcholanthrene (3MC).

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 492492Cytochrome P450 2A1

Sites

Metal binding4371Iron (heme axial ligand)

Experimental info

Sequence conflict3861G → A in AAA41030. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P11711-1 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: 58A187F9F10A1D85

FASTA49255,995
        10         20         30         40         50         60 
MLDTGLLLVV ILASLSVMLL VSLWQQKIRG RLPPGPTPLP FIGNYLQLNT KDVYSSITQL 

        70         80         90        100        110        120 
SERYGPVFTI HLGPRRVVVL YGYDAVKEAL VDQAEEFSGR GEQATYNTLF KGYGVAFSSG 

       130        140        150        160        170        180 
ERAKQLRRLS IATLRDFGVG KRGVEERILE EAGYLIKMLQ GTCGAPIDPT IYLSKTVSNV 

       190        200        210        220        230        240 
ISSIVFGERF DYEDTEFLSL LQMMGQMNRF AASPTGQLYD MFHSVMKYLP GPQQQIIKVT 

       250        260        270        280        290        300 
QKLEDFMIEK VRQNHSTLDP NSPRNFIDSF LIRMQEEKNG NSEFHMKNLV MTTLSLFFAG 

       310        320        330        340        350        360 
SETVSSTLRY GFLLLMKHPD VEAKVHEEIE QVIGRNRQPQ YEDHMKMPYT QAVINEIQRF 

       370        380        390        400        410        420 
SNLAPLGIPR RIIKNTTFRG FFLPKGTDVF PILGSLMTDP KFFPSPKDFD PQNFLDDKGQ 

       430        440        450        460        470        480 
LKKNAAFLPF STGKRFCLGD GLAKMELFLL LTTILQNFRF KFPMKLEDIN ESPKPLGFTR 

       490 
IIPKYTMSFM PI

« Hide

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References

« Hide 'large scale' references
[1]"Rat testosterone 7 alpha-hydroxylase. Isolation, sequence, and expression of cDNA and its developmental regulation and induction by 3-methylcholanthrene."
Nagata K., Matsunaga T., Gillette J., Gelboin H.V., Gonzalez F.J.
J. Biol. Chem. 262:2787-2793(1987) [PubMed: 3818621] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7."
Matsunaga T., Nomoto M., Kozak C.A., Gonzalez F.J.
Biochemistry 29:1329-1341(1990) [PubMed: 2322568] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]"Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity."
Arlotto M.P., Greenway D.J., Parkinson A.
Arch. Biochem. Biophys. 270:441-457(1989) [PubMed: 2650624] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-20.
Tissue: Liver.
[5]"Purification and characterization of six cytochromes P-450 from hepatic microsomes of immature female rats."
Imaoka S., Kamataki T., Funae Y.
J. Biochem. 102:843-851(1987) [PubMed: 3436956] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-12.
Tissue: Liver.

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Cross-references

Sequence databases

J02669 mRNA. Translation: AAA41030.1.
M33312 Genomic DNA. Translation: AAA41020.1.
BC081848 mRNA. Translation: AAH81848.1.
PIRA34272.
RefSeqNP_036824.1.
UniGeneRn.32107

3D structure databases

HSSPHSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBaseSearch...

Genome annotation databases

EnsemblENSRNOG00000020928. Rattus norvegicus. [Contig view]
GeneID24894.
KEGGrno:24894.

Organism-specific databases

RGD2463. Cyp2a1.

Phylogenomic databases

HOVERGENP11711.

Gene expression databases

ArrayExpressP11711.
GermOnlineENSRNOG00000020928. Rattus norvegicus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomP11711.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameCP2A1_RAT
AccessionPrimary (citable) accession number: P11711
Secondary accession number(s): Q642C5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 1, 2007
Last modified: September 2, 2008
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents