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Reviewed, UniProtKB/Swiss-Prot P10612 (CP11A_PIG)

Last modified July 22, 2008. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 11A1, mitochondrial
    EC=1.14.15.6
Alternative name(s):
    CYPXIA1
    Cholesterol side-chain cleavage enzyme
    P450(scc)
    Cholesterol desmolase
Gene names
Name: CYP11A1
OrganismSus scrofa (Pig)
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

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Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

Catalytic activity

Cholesterol + reduced adrenal ferredoxin + O(2) = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H(2)O.

Cofactor

Heme group By similarity.

Pathway

Lipid metabolism; C21-steroid hormone metabolism.

Subcellular location

Mitochondrion membrane.

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Biological processCholesterol metabolism
Lipid metabolism
Steroid metabolism
Steroidogenesis
   Cellular componentMembrane
Mitochondrion
   DomainTransit peptide
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Cellular componentmitochondrial membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncholesterol monooxygenase (side-chain-cleaving) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Transit peptide1 – 3939Mitochondrion
Chain40 – 520481Cytochrome P450 11A1, mitochondrial

Sites

Metal binding4611Iron (heme axial ligand)

Experimental info

Sequence conflict461R → F AA sequence Ref.3

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Sequences

Sequence LengthMass (Da)Tools
P10612-1 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 6C6D0D6FE34F9C33

FASTA52060,258
        10         20         30         40         50         60 
MLARGLALRS VLVKGCQPFL SAPRECPGHP RVGTGEGACI STKTPRPFSE IPSPGDNGWI 

        70         80         90        100        110        120 
NLYRFWKEKG TQKIHYHHVQ NFQKYGPIYR EKLGNLESVY IIDPEDVALL FKFEGPNPER 

       130        140        150        160        170        180 
YNIPPWVAYH QHYQKPVGVL LKKSGAWKKD RLVLNTEVMA PEAIKNFIPL LDTVSQDFVG 

       190        200        210        220        230        240 
VLHRRIKQQG SGKFSGDIRE DLFRFAFESI TNVIFGERLG MLEEIVDPEA QKFIDAVYQM 

       250        260        270        280        290        300 
FHTSVPMLNL PPDLFRLFRT KTWRDHVAAW DTIFNKAEKY TQNFYWDLRR KREFNNYPGI 

       310        320        330        340        350        360 
LYRLLGNDKL LSEDVKANVT EMLAGGVDTT SMTLQWHLYE MARSLNVQEM LREEVLNARR 

       370        380        390        400        410        420 
QAQGDTSKML QLVPLLKASI KETLRLHPIS VTLQRYLVND LVLRDYMIPA KTLVQVAVYA 

       430        440        450        460        470        480 
MGRDPAFFSN PGQFDPTRWL GKERDLIHFR NLGFGWGVRQ CVGRRIAELE MTLFLIHILE 

       490        500        510        520 
NFKVELQHFS DVDTIFNLIL MPDKPIFLVF RPFNQDPLQA

« Hide

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References

[1]"Nucleotide sequence of cytochrome P-450 cholesterol side-chain cleavage cDNA isolated from porcine testis."
Mulheron G.W., Stone R.T., Miller W.L., Wise T.H.
Nucleic Acids Res. 17:1773-1773(1989) [PubMed: 2922297] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[2]"Purification and properties of cytochrome P-450 (SCC) from pig testis mitochondria."
Kuwada M., Kitajima R., Suzuki H., Horie S.
Biochem. Biophys. Res. Commun. 176:1501-1508(1991) [PubMed: 2039527] [Abstract]
Cited for: PROTEIN SEQUENCE OF 41-61.
Tissue: Testis.
[3]"Purification and comparative characterization of cytochrome P-450scc from porcine adrenocortical mitochondria."
Iwahashi K., Tsubaki M., Miyatake A., Ichikawa Y.
Int. J. Biochem. 23:901-909(1991) [PubMed: 1773895] [Abstract]
Cited for: PROTEIN SEQUENCE OF 40-64.

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Cross-references

Sequence databases

X13768 mRNA. Translation: CAA32018.1.
PIRS03188.
RefSeqNP_999592.1.
UniGeneSsc.8776

3D structure databases

HSSPHSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBaseSearch...

Genome annotation databases

GeneID403329.
KEGGssc:403329.

Phylogenomic databases

HOVERGENP10612.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomP10612.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameCP11A_PIG
AccessionPrimary (citable) accession number: P10612
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 22, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents