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Reviewed, UniProtKB/Swiss-Prot P05183 (CP3A2_RAT)

Last modified September 2, 2008. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 3A2
    EC=1.14.14.1
Alternative name(s):
    CYPIIIA2
    P450-PCN2
    P450/6-beta-A
    Testosterone 6-beta-hydroxylase
Gene names
Name: Cyp3a2
Synonyms: Cyp3a-2, Cyp3a11
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Expressed in liver.

Induction

By pregnenolone 16-alpha-carbonitrile (PNCN) and dexamethasone.

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 504504Cytochrome P450 3A2

Sites

Metal binding4431Iron (heme axial ligand)

Experimental info

Sequence conflict56 – 572GL → AV in CAA55887. Ref.2
Sequence conflict4291D → H in AAA41051. Ref.1
Sequence conflict4451G → D in AAA41051. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P05183-1 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: C2C84AF736035AB2

FASTA50457,732
        10         20         30         40         50         60 
MDLLSALTLE TWVLLAVILV LLYRLGTHRH GIFKKQGIPG PKPLPFLGTV LNYYKGLGRF 

        70         80         90        100        110        120 
DMECYKKYGK IWGLFDGQTP VFAIMDTEMI KNVLVKECFS VFTNRRDFGP VGIMGKAVSV 

       130        140        150        160        170        180 
AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE QYGDILVKYL KQEAETGKPV TMKKVFGAYS 

       190        200        210        220        230        240 
MDVITSTSFG VNVDSLNNPK DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM 

       250        260        270        280        290        300 
FPKDSIAFFQ KFVHRIKETR LDSKHKHRVD FLQLMLNAHN NSKDEVSHKA LSDVEIIAQS 

       310        320        330        340        350        360 
VIFIFAGYET TSSTLSFVLY FLATHPDIQK KLQEEIDGAL PSKAPPTYDI VMEMEYLDMV 

       370        380        390        400        410        420 
LNETLRLYPI GNRLERVCKK DIELDGLFIP KGSVVTIPTY ALHHDPQHWP KPEEFHPERF 

       430        440        450        460        470        480 
SKENKGSIDP YVYLPFGNGP RNCIGMRFAL MNMKLALTKV LQNFSFQPCK ETQIPLKLSR 

       490        500 
QAILEPEKPI VLKVLPRDAV INGA

« Hide

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References

« Hide 'large scale' references
[1]"Pregnenolone 16 alpha-carbonitrile-inducible P-450 gene family: gene conversion and differential regulation."
Gonzalez F.J., Song B.-J., Hardwick J.P.
Mol. Cell. Biol. 6:2969-2976(1986) [PubMed: 3785219] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A gene structure of testosterone 6 beta-hydroxylase (P450IIIA)."
Miyata M., Nagata K., Yamazoe Y., Kato R.
Biochem. Biophys. Res. Commun. 177:68-73(1991) [PubMed: 2043144] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[3]"Structure of a gene and cDNA of a major constitutive form of testosterone 6 beta-hydroxylase (P450/6 beta A) encoding CYP3A2: comparison of the cDNA with P450PCN2."
Miyata M., Nagata K., Shimada M., Yamazoe Y., Kato R.
Arch. Biochem. Biophys. 314:351-359(1994) [PubMed: 7979376] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[5]"Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development."
Telhada M.B., Pereira T.M., Lechner M.C.
Arch. Biochem. Biophys. 298:715-725(1992) [PubMed: 1417000] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-36, INDUCTION.
Tissue: Liver.
[6]"Purification and characterization of four catalytically active testosterone 6 beta-hydroxylase P-450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms."
Nagata K., Gonzalez F.J., Yamazoe Y., Kato R.
J. Biochem. 107:718-725(1990) [PubMed: 2398038] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-33, TISSUE SPECIFICITY, INDUCTION.
Tissue: Liver.
[7]"Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats."
Cooper K.O., Reik L.M., Jayyosi Z., Bandiera S., Kelley M., Ryan D.E., Daniel R., McCluskey S.A., Levin W., Thomas P.E.
Arch. Biochem. Biophys. 301:345-354(1993) [PubMed: 7681660] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-25, TISSUE SPECIFICITY, INDUCTION.

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Cross-references

Sequence databases

M13646 mRNA. Translation: AAA41051.1.
X79319 Genomic DNA. Translation: CAA55887.1.
X79320 mRNA. Translation: CAA55888.1.
U09742 mRNA. Translation: AAA82168.1.
U09734 expand/collapse EMBL AC list , U09725, U09726, U09727, U09728, U09729, U09730, U09731, U09732, U09733 Genomic DNA. Translation: AAB60492.1.
BC089765 mRNA. Translation: AAH89765.1.
PIRA25222.
RefSeqNP_695224.2.
UniGeneRn.202943

3D structure databases

HSSPHSSP built from PDB template 1JPZ based on UniProtKB P14779.
SMRP05183. Positions 28-499.
ModBaseSearch...

Genome annotation databases

EnsemblENSRNOG00000000978. Rattus norvegicus. [Contig view]
GeneID266682.
KEGGrno:266682.

Organism-specific databases

RGD708379. Cyp3a2.

Phylogenomic databases

HOVERGENP05183.

Gene expression databases

ArrayExpressP05183.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR008072. Cyt_P450_E_CYP3A.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01689. EP450IICYP3A.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomP05183.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameCP3A2_RAT
AccessionPrimary (citable) accession number: P05183
Secondary accession number(s): Q5FVU5, Q64629, Q64672
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 15, 1999
Last modified: September 2, 2008
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents