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Reviewed, UniProtKB/Swiss-Prot P04800 (CP3A1_RAT)

Last modified September 2, 2008. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 3A1
    EC=1.14.14.1
Alternative name(s):
    CYPIIIA1
    P450-PCN1
Gene names
Name: Cyp3a1
Synonyms: Cyp3a-1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

By pregnenolone 16-alpha-carbonitrile (PNCN).

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityPolymorphism
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 504504Cytochrome P450 3A1

Sites

Metal binding4431Iron (heme axial ligand)

Natural variations

Natural variant2071T → A in strain: Wistar.
Natural variant2131F → I in strain: Wistar.
Natural variant2321I → V in strain: Wistar.

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Sequences

Sequence LengthMass (Da)Tools
P04800-1 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: CFD5AC8C37E9CADB

FASTA50457,918
        10         20         30         40         50         60 
MDLLSALTLE TWVLLAVVLV LLYGFGTRTH GLFKKQGIPG PKPLPFFGTV LNYYMGLWKF 

        70         80         90        100        110        120 
DVECHKKYGK IWGLFDGQMP LFAITDTEMI KNVLVKECFS VFTNRRDFGP VGIMGKAVSV 

       130        140        150        160        170        180 
AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE QYGDILVKYL KQEAETGKPV TMKKVFGAYS 

       190        200        210        220        230        240 
MDVITSTSFG VNVDSLNNPK DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM 

       250        260        270        280        290        300 
FPKDSIEFFK KFVYRMKETR LDSVQKHRVD FLQLMMNAHN DSKDKESHTA LSDMEITAQS 

       310        320        330        340        350        360 
IIFIFAGYEP TSSTLSFVLH SLATHPDTQK KLQEEIDRAL PNKAPPTYDT VMEMEYLDMV 

       370        380        390        400        410        420 
LNETLRLYPI GNRLERVCKK DVEINGVFMP KGSVVMIPSY ALHRDPQHWP EPEEFRPERF 

       430        440        450        460        470        480 
SKENKGSIDP YVYLPFGNGP RNCIGMRFAL MNMKLALTKV LQNFSFQPCK ETQIPLKLSR 

       490        500 
QGLLQPTKPI ILKVVPRDEI ITGS

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References

[1]"Complete cDNA and protein sequence of a pregnenolone 16 alpha-carbonitrile-induced cytochrome P-450. A representative of a new gene family."
Gonzalez F.J., Nebert D.W., Hardwick J.P., Kasper C.B.
J. Biol. Chem. 260:7435-7441(1985) [PubMed: 3838989] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and characterization of a novel CYP3A1 allelic variant: analysis of CYP3A1 and CYP3A2 sex-hormone-dependent expression reveals that the CYP3A2 gene is regulated by testosterone."
Ribeiro V., Lechner M.C.
Arch. Biochem. Biophys. 293:147-152(1992) [PubMed: 1731631] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Liver.
[3]"Purification and characterization of four catalytically active testosterone 6 beta-hydroxylase P-450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms."
Nagata K., Gonzalez F.J., Yamazoe Y., Kato R.
J. Biochem. 107:718-725(1990) [PubMed: 2398038] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-26.
[4]"Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats."
Cooper K.O., Reik L.M., Jayyosi Z., Bandiera S., Kelley M., Ryan D.E., Daniel R., McCluskey S.A., Levin W., Thomas P.E.
Arch. Biochem. Biophys. 301:345-354(1993) [PubMed: 7681660] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-25.
[5]"Paradoxical transcriptional activation of rat liver cytochrome P-450 3A1 by dexamethasone and the antiglucocorticoid pregnenolone 16 alpha-carbonitrile: analysis by transient transfection into primary monolayer cultures of adult rat hepatocytes."
Burger H.J., Schuetz J.D., Schuetz E.G., Guzelian P.S.
Proc. Natl. Acad. Sci. U.S.A. 89:2145-2149(1992) [PubMed: 1372436] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
[6]"Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development."
Telhada M.B., Pereira T.M., Lechner M.C.
Arch. Biochem. Biophys. 298:715-725(1992) [PubMed: 1417000] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-24.
Tissue: Liver.

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Cross-references

Sequence databases

M10161 mRNA. Translation: AAA41035.1.
X64401 mRNA. Translation: CAA45743.1.
M86850 Genomic DNA. Translation: AAA41780.1.
X62086 Genomic DNA. No translation available.
PIRA22631.
UniGeneRn.91120

3D structure databases

HSSPHSSP built from PDB template 1JPZ based on UniProtKB P14779.
SMRP04800. Positions 29-498.
ModBaseSearch...

PTM databases

PhosphoSiteP04800.

Genome annotation databases

EnsemblENSRNOG00000032560. Rattus norvegicus. [Contig view]

Organism-specific databases

RGD628626. Cyp3a1.

Phylogenomic databases

HOVERGENP04800.

Gene expression databases

ArrayExpressP04800.
GermOnlineENSRNOG00000032560. Rattus norvegicus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR008072. Cyt_P450_E_CYP3A.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01689. EP450IICYP3A.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomP04800.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameCP3A1_RAT
AccessionPrimary (citable) accession number: P04800
Secondary accession number(s): Q64580
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 2, 2008
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents