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Reviewed, UniProtKB/Swiss-Prot P03940 (CP21A_MOUSE)

Last modified September 2, 2008. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 21
    EC=1.14.99.10
Alternative name(s):
    Cytochrome P450 XXI
    Steroid 21-hydroxylase
    21-OHase
    P450-C21
    P-450c21
Gene names
Name: Cyp21
Synonyms: Cyp21a-1, Cyp21a1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activity

A steroid + AH(2) + O(2) = a 21-hydroxysteroid + A + H(2)O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Domain

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Biological processSteroidogenesis
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Lipid-binding
Metal-binding
Steroid-binding
   Molecular functionMonooxygenase
Oxidoreductase

Gene Ontology (GO)

None. [Check GOA]

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 487487Cytochrome P450 21

Regions

Region334 – 35017Steroid-binding By similarity

Sites

Metal binding4201Iron (heme axial ligand)

Experimental info

Sequence conflict31 – 322PP → LR in AAA37114. Ref.1
Sequence conflict661M → L in AAA37114. Ref.1
Sequence conflict2181L → F in BAA31153. Ref.3
Sequence conflict2381Q → R Ref.3 Ref.4
Sequence conflict2411D → E in AAA37114. Ref.1
Sequence conflict3141E → A in AAA37114. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P03940-1 [UniParc].

Last modified April 26, 2005. Version 3.
Checksum: CAD32B606004449F

FASTA48755,328
        10         20         30         40         50         60 
MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL GLTQKLGPIY 

        70         80         90        100        110        120 
RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM DLDLSLGDYS LMWKAHKKLS 

       130        140        150        160        170        180 
RSALMLGMRD SMEPLIEQLT QEFCERMRAQ AGTPVAIHKE FSFLTCSIIS CLTFGDKDST 

       190        200        210        220        230        240 
LVQTLHDCVQ DLLQAWNHWS IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK 

       250        260        270        280        290        300 
DSLVAGQWKD MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA 

       310        320        330        340        350        360 
FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV VPLALPHRAT 

       370        380        390        400        410        420 
RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR FLEPGKNPRT PSFGCGARVC 

       430        440        450        460        470        480 
LGEPLARLEL FVVLARLLQA FTLLPPPDGT LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP 


QDQGERP

« Hide

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References

« Hide 'large scale' references
[1]"Nucleotide sequence analysis of murine 21-hydroxylase genes: mutations affecting gene expression."
Chaplin D.D., Galbraith L.J., Seidman J.G., White P.C., Parker K.L.
Proc. Natl. Acad. Sci. U.S.A. 83:9601-9605(1986) [PubMed: 3491986] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The murine Slp gene: additional evidence that sex-limited protein has no biological function."
Zepf N.E., Ogata R.T.
J. Immunol. 147:2756-2763(1991) [PubMed: 1918990] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: B10.WR.
[3]"Mus musculus 5' truncated pseudogene of tenascin-X, steroid 21-hydroxylase (Cyp21), and sex-limited protein (Slp) genes, partial cds and complete sequences."
Matsumoto K., Ikuta T.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C57BL/6 X CBA.
[4]"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
Genome Res. 13:2621-2636(2003) [PubMed: 14656967] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 129.
[5]"Liver mRNA probes disclose two cytochrome P-450 genes duplicated in tandem with the complement C4 loci of the mouse H-2S region."
Amor M., Tosi M., Duponchel C., Steinmetz M., Meo T.
Proc. Natl. Acad. Sci. U.S.A. 82:4453-4457(1985) [PubMed: 3874401] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-487.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M15009 Genomic DNA. Translation: AAA37114.1.
M64933 Genomic DNA. Translation: AAA40118.1.
AB015623 Genomic DNA. Translation: BAA31153.1.
AF049850 Genomic DNA. Translation: AAC05278.1.
PIRA26660.
S54785.
UniGeneMm.440407

3D structure databases

HSSPHSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBaseSearch...

Genome annotation databases

EnsemblENSMUSG00000024365. Mus musculus. [Contig view]

Organism-specific databases

MGIMGI:88591. Cyp21a1.

Phylogenomic databases

HOVERGENP03940.

Gene expression databases

ArrayExpressP03940.
GermOnlineENSMUSG00000024365. Mus musculus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomP03940.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

SOURCESearch...
ProtoNetSearch...

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Entry information

Entry nameCP21A_MOUSE
AccessionPrimary (citable) accession number: P03940
Secondary accession number(s): O88304, Q64510, Q9QX14
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: April 26, 2005
Last modified: September 2, 2008
This is version 72 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents