Outer membrane protein tolC precursor - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P02930 (TOLC_ECOLI)

Last modified September 2, 2008. Version 93. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Outer membrane protein tolC

Gene names

Name:	tolC
Synonyms:	mtcB, mukA, refI
Ordered Locus Names:	b3035, JW5503

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria.
Subunit structure	Homotrimer that assembles to form a continuous, solvent-accessible conduit: a 'channel-tunnel' over 140 Angstroms long that spans both the outer membrane and periplasmic space. The periplasmic or proximal end of the tunnel is sealed by sets of coiled helices.
Subcellular location	Cell outer membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the outer membrane factor (OMF) (TC 1.B.17) family. [View classification]
Sequence caution	The sequence CAA24914.1 differs from that shown. Reason: Miscellaneous discrepancy.

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Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Repeat Signal Transmembrane
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Signal peptide

1 – 22

Chain

23 – 493

471

Outer membrane protein tolC

Regions

Topological domain

23 – 62

Periplasmic

Transmembrane

63 – 74

Topological domain

75 – 82

Extracellular

Transmembrane

83 – 96

Topological domain

97 – 268

172

Periplasmic

Transmembrane

269 – 279

Topological domain

280 – 300

Extracellular

Transmembrane

301 – 311

Topological domain

312 – 493

182

Periplasmic

Repeat

23 – 230

208

Repeat

231 – 446

216

Experimental info

Sequence conflict

191

V → L in CAA37982. Ref.1

Secondary structure

493

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P02930-1 [UniParc].

Last modified June 27, 2006. Version 3.
Checksum: 6F97B4C62A848FE1
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FASTA

493

53,741

        10         20         30         40         50         60 
MKKLLPILIG LSLSGFSSLS QAENLMQVYQ QARLSNPELR KSAADRDAAF EKINEARSPL 

        70         80         90        100        110        120 
LPQLGLGADY TYSNGYRDAN GINSNATSAS LQLTQSIFDM SKWRALTLQE KAAGIQDVTY 

       130        140        150        160        170        180 
QTDQQTLILN TATAYFNVLN AIDVLSYTQA QKEAIYRQLD QTTQRFNVGL VAITDVQNAR 

       190        200        210        220        230        240 
AQYDTVLANE VTARNNLDNA VEQLRQITGN YYPELAALNV ENFKTDKPQP VNALLKEAEK 

       250        260        270        280        290        300 
RNLSLLQARL SQDLAREQIR QAQDGHLPTL DLTASTGISD TSYSGSKTRG AAGTQYDDSN 

       310        320        330        340        350        360 
MGQNKVGLSF SLPIYQGGMV NSQVKQAQYN FVGASEQLES AHRSVVQTVR SSFNNINASI 

       370        380        390        400        410        420 
SSINAYKQAV VSAQSSLDAM EAGYSVGTRT IVDVLDATTT LYNAKQELAN ARYNYLINQL 

       430        440        450        460        470        480 
NIKSALGTLN EQDLLALNNA LSKPVSTNPE NVAPQTPEQN AIADGYAPDS PAPVVQQTSA 

       490 
RTTTSNGHNP FRN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of the tolC gene that codes for an outer membrane protein of Escherichia coli K12." Hackett J., Reeves P. Nucleic Acids Res. 11:6487-6495(1983) [PubMed: 6312426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the tolC gene of Escherichia coli." Niki H., Imamura R., Ogura T., Hiraga S. Nucleic Acids Res. 18:5547-5547(1990) [PubMed: 2216730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The TolC protein of Escherichia coli K12 is synthesised in a precursor form." Hackett J., Misra R., Reeves P. FEBS Lett. 156:307-310(1983) [PubMed: 6303857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45. Strain: K12.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-34. Strain: K12 / EMG2.
[7]	"Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis." Molloy M.P., Herbert B.R., Walsh B.J., Tyler M.I., Traini M., Sanchez J.-C., Hochstrasser D.F., Williams K.L., Gooley A.A. Electrophoresis 19:837-844(1998) [PubMed: 9629924] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-27. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Protein complexes of the Escherichia coli cell envelope." Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O. J. Biol. Chem. 280:34409-34419(2005) [PubMed: 16079137] [Abstract] Cited for: SUBUNIT, SUBCELLULAR LOCATION. Strain: BL21-DE3.
[9]	"Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export." Koronakis V., Sharff A., Koronakis E., Luisi B., Hughes C. Nature 405:914-919(2000) [PubMed: 10879525] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 23-450.

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Cross-references

Sequence databases

X00016 Genomic DNA. Translation: CAA24914.1. Sequence problems.
X54049 Genomic DNA. Translation: CAA37982.1. Different initiation.
U28377 Genomic DNA. Translation: AAA69203.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76071.2.
AP009048 Genomic DNA. Translation: BAE77091.1.
V01505 Genomic DNA. Translation: CAA24751.1. Different initiation.

PIR

MMECTC. A65091.

RefSeq

AP_003585.1.
NP_417507.2.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EK9	X-ray	2.10	A/B/C	23-450	[»]
1TQQ	X-ray	2.75	A/B/C	23-493	[»]
1TTQ	X-ray	2.00	A	-	[»]
			B	1-12	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:11007N.

2-D gel databases

SWISS-2DPAGE

P02930.

2DBase-Ecoli

P02930.

Genome annotation databases

GeneID

947521.

GenomeReviews

Gene locus b3035 in contig U00096_GR.
Gene locus JW5503 in contig AP009048_GR.

KEGG

ecj:JW5503.
eco:b3035.

Organism-specific databases

EchoBASE

EB1002.

EcoGene

EG11009. tolC.

CMR

Search...

Phylogenomic databases

HOGENOM

P02930.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11009-MON.

Family and domain databases

InterPro

IPR003423. OMP_efflux.
IPR010130. T1SS_OMP_TolC.
[Graphical view]

Pfam

PF02321. OEP. 2 hits.
[Graphical view]

TIGRFAMs

TIGR01844. type_I_sec_TolC. 1 hit.

ProDom

P02930.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

LinkHub

P02930.

ProtoNet

Search...

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Entry information

Entry name

TOLC_ECOLI

Accession

Primary (citable) accession number: P02930
Secondary accession number(s): Q2M9G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 27, 2006
Last modified:	September 2, 2008
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Recent format changes

Overview of recent format changes

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2-D gel databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other Resources

Entry information

Relevant documents