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Reviewed, UniProtKB/Swiss-Prot P00191 (CP21A_BOVIN)

Last modified September 2, 2008. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome P450 21
    EC=1.14.99.10
Alternative name(s):
    Cytochrome P450 XXI
    Steroid 21-hydroxylase
    21-OHase
    P450-C21
    P-450c21
Gene names
Name: CYP21
Synonyms: CYP21A1
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activity

A steroid + AH(2) + O(2) = a 21-hydroxysteroid + A + H(2)O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Domain

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similarities

Belongs to the cytochrome P450 family.

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Ontologies

Keywords

   Biological processSteroidogenesis
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Lipid-binding
Metal-binding
Steroid-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 496496Cytochrome P450 21

Regions

Region341 – 35717Steroid-binding By similarity

Sites

Metal binding4271Iron (heme axial ligand)

Experimental info

Sequence conflict131L → K AA sequence Ref.4
Sequence conflict141A → S in AAA30487. Ref.2
Sequence conflict4011H → Y in AAA30487. Ref.2
Sequence conflict4311S → C in AAA30486. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
P00191-1 [UniParc].

Last modified November 1, 1990. Version 2.
Checksum: 2E87C829A7E66B31

FASTA49656,077
        10         20         30         40         50         60 
MVLAGLLLLL TLLAGAHLLW GRWKLRNLHL PPLVPGFLHL LQPNLPIHLL SLTQKLGPVY 

        70         80         90        100        110        120 
RLRLGLQEVV VLNSKRTIEE AMIRKWVDFA GRPQIPSYKL VSQRCQDISL GDYSLLWKAH 

       130        140        150        160        170        180 
KKLTRSALLL GTRSSMEPWV DQLTQEFCER MRVQAGAPVT IQKEFSLLTC SIICYLTFGN 

       190        200        210        220        230        240 
KEDTLVHAFH DCVQDLMKTW DHWSIQILDM VPFLRFFPNP GLWRLKQAIE NRDHMVEKQL 

       250        260        270        280        290        300 
TRHKESMVAG QWRDMTDYML QGVGRQRVEE GPGQLLEGHV HMSVVDLFIG GTETTASTLS 

       310        320        330        340        350        360 
WAVAFLLHHP EIQRRLQEEL DRELGPGASC SRVTYKDRAR LPLLNATIAE VLRLRPVVPL 

       370        380        390        400        410        420 
ALPHRTTRPS SIFGYDIPEG MVVIPNLQGA HLDETVWEQP HEFRPDRFLE PGANPSALAF 

       430        440        450        460        470        480 
GCGARVCLGE SLARLELFVV LLRLLQAFTL LPPPVGALPS LQPDPYCGVN LKVQPFQVRL 

       490 
QPRGVEAGAW ESASAQ

« Hide

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References

[1]"Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family."
Chung B., Matteson K.J., Miller W.L.
Proc. Natl. Acad. Sci. U.S.A. 83:4243-4247(1986) [PubMed: 3487086] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Structural analysis of cloned cDNA for mRNA of microsomal cytochrome P-450(C21) which catalyzes steroid 21-hydroxylation in bovine adrenal cortex."
Yoshioka H., Morohashi K., Sogawa K., Yamane M., Kominami S., Takemori S., Okada Y., Omura T., Fujii-Kuriyama Y.
J. Biol. Chem. 261:4106-4109(1986) [PubMed: 3005319] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Bovine steroid 21-hydroxylase: regulation of biosynthesis."
John M.E., Okamura T., Dee A., Adler B., John M.C., White P.C., Simpson E.R., Waterman M.R.
Biochemistry 25:2846-2853(1986) [PubMed: 2424492] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 121-496.
[4]"Partial amino acid sequences of two mitochondrial and two microsomal cytochrome P-450's from adrenal cortex."
Ogishima T., Okada Y., Kominami S., Takemori S., Omura T.
J. Biochem. 94:1711-1714(1983) [PubMed: 6654880] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-15.

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Cross-references

Sequence databases

M11267 Genomic DNA. Translation: AAA83247.1.
M12918 mRNA. Translation: AAA30487.1.
K01333 mRNA. Translation: AAA30486.1.
PIRO4BOC2. A27555.
RefSeqNP_001013614.1.
NP_777064.1.
UniGeneBt.1964

3D structure databases

HSSPHSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBaseSearch...

Genome annotation databases

EnsemblENSBTAG00000001443. Bos taurus. [Contig view]
GeneID281741.
282425.
KEGGbta:281741.
bta:282425.

Phylogenomic databases

HOVERGENP00191.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProDomP00191.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameCP21A_BOVIN
AccessionPrimary (citable) accession number: P00191
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1990
Last modified: September 2, 2008
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents