Isolation, molecular characterization, and tissue-specific expression of a novel putative G protein-coupled receptor.
Mayer H., Salzer U., Breuss J., Ziegler S., Marchler-Bauer A., Prohaska R.
We isolated a 40 kDa integral membrane protein (p40) from human erythrocyte ghosts by affinity chromatography, using a C-terminal peptide of stomatin, and obtained partial sequences which enabled us to isolate two full-length cDNAs from human bone marrow and fetal brain cDNA libraries. The cDNA sequences were identical and encoded a novel putative G protein-coupled receptor (399 amino acids). Northern and RNA dot blot analyses demonstrated that the major 4.8 kb-transcript is predominantly expressed in brain. In situ hybridization studies of tissue sections revealed high expression in neurons of the brain and spinal cord, in thymocytes, megakaryocytes, and macrophages.
Biochim. Biophys. Acta 1395:301-308(1998) [PubMed] [Europe PMC]