Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen.
Robert L.S., Allard S., Gerster J.L., Cass L., Simmonds J.
A cDNA clone, Sta 44-4, corresponding to a mRNA highly expressed in Brassica napus cv. Westar stamens, was isolated by differential screening and characterized. Northern blot and in situ analyses demonstrated that Sta 44-4 is synthesized in pollen beginning at the late uninucleate stage and reaches a maximum in trinucleate microspores. Sta 44-4 displayed significant sequence similarity to known pollen polygalacturonase genes. The B. napus pollen polygalacturonase gene was shown to be part of a small gene family and to display some polymorphism among different cultivars.
Plant Mol. Biol. 23:1273-1278(1993) [ PubMed | SRS | CiteXplore ]
