Cloning, sequence and structural features of a lipase from the antarctic facultative psychrophile Psychrobacter immobilis B10.
Arpigny J.L., Feller G., Gerday C.
A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35,288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold.
Biochim. Biophys. Acta 1171:331-333(1993) [ PubMed | SRS | CiteXplore ]
