Molecular cloning and nucleotide sequence of the beta-galactosidase gene from Enterobacter cloacae GAO.
Nagano H., Kawaguchi T., Omori M., Shoji Z., Arai M.
The gene encoding a beta-galactosidase from Enterobacter cloacae GAO was cloned and expressed in Escherichia coli. The nucleotide sequence of the insert of a positive clone had an open reading frame of 3084 bp that encoded a polypeptide of 1028 amino acid residues with a calculated molecular mass of 116,677 daltons. The amino acid sequence of beta-galactosidase deduced from the nucleotide sequence, especially the sequence around the putative active site and of the fourteen regions, showed significant homology to beta-galactosidases of other microorganisms, E. coli, Klebsiella pneumoniae, Lactobacillus bulgaricus, and Clostridium acetobutylicum.
Biosci. Biotechnol. Biochem. 58:1866-1869(1994) [ PubMed | SRS | CiteXplore ]
