Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene.
Vrijbloed J.W., van Hylckama Vlieg J., van der Put N.M., Hessels G.I., Dijkhuizen L.
An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E. coli and characterized.
J. Bacteriol. 177:6666-6669(1995) [ PubMed | SRS | CiteXplore ]
