The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene.
Swindle J., Ajioka J., Dawson D., Myers R., Carroll D., Georgopoulos C.
The nusB (groNB) gene product of Escherichia coli plays a pivotal role in allowing bacteriophage lambda N protein to function as an antiterminator of mRNA transcription and in modulating host gene expression. In addition it is essential for bacterial viability since mutations in it result in a cold-sensitivity phenotype for growth. We have previously cloned the nusB gene and shown it to code for a 14,500-Mr protein. Here we present the primary DNA sequence of the nusB gene. From the sequence we deduce that it codes for a slightly basic protein (21 basic as opposed to 20 acidic amino acids) composed of 139 amino acids with a cumulative 15,689-Mr. The predicted N-terminal amino acid sequence as well as the overall amino acid composition agrees well with that of the purified protein.
Nucleic Acids Res. 12:4977-4985(1984) [ PubMed | SRS | CiteXplore ]
