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Two forms of pyruvate kinase in Escherichia coli. A comparison of chemical and molecular properties.

Valentini G., Iadarola P., Somani B.L., Malcovati M.

The two forms of pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC present in Escherichia coli have been purified from the same cultures and crystallized. A modified procedure for the purification of type I pyruvate kinase is described. Molecular weight, subunit structure, amino acid composition, NH2-terminal amino acid, maps of tryptic peptides and conditions for crystallization have been determined for the two forms. A comparison of these data shows that the two forms are different proteins, each being a tetramer of identical subunits.

Biochim. Biophys. Acta 570:248-258(1979) [PubMed] [Europe PMC]