Cloning and sequencing the HinfI restriction and modification genes.
Chandrasegaran S., Lunnen K.D., Smith H.O., Wilson G.G.
The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).
Gene 70:387-392(1988) [ PubMed | SRS | CiteXplore ]
