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The primary structure of porcine liver acylamino acid-releasing enzyme deduced from cDNA sequences.

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Mitta M., Asada K., Uchimura Y., Kimizuka F., Kato I., Sakiyama F., Tsunasawa S.

Two overlapping cloned cDNAs encoding the entire amino acid sequences of the subunits of acylamino acid-releasing enzyme (AARE) [EC 3.4.19.1] have been isolated from porcine liver cDNA lambda gt10 cDNA libraries and sequenced. Sequence analyses of the cDNA and several Achromobacter protease I-digested peptides of the purified protein revealed that porcine liver AARE consists of four identical subunits, and each comprising a single chain of 732 amino acids with acetylmethionine at the N-terminus.

J. Biochem. 106:548-551(1989) [ PubMed | SRS | CiteXplore ]