Cloning and DNA sequence analysis of a polygalacturonase cDNA from Aspergillus niger RH5344.
Ruttowski E., Labitzke R., Khanh N.Q., Loeffler F., Gottschalk M., Jany K.-D.
A 1319 bp long cDNA encoding for a polygalacturonase (EC 3.2.1.15) from Aspergillus niger RH5344 comprises a single open reading frame of 1089 bp which includes the mature protein of 362 amino acids and an NH2-terminal signal peptide of 27 amino acids. The directly determined peptides of the mature polygalacturonase confirmed the sequence information deduced from the cDNA.
Biochim. Biophys. Acta 1087:104-106(1990) [ PubMed | SRS | CiteXplore ]
