Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.

Cho S.W., Kim S., Kim J.M., Kim J.S.

We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.

Nat. Biotechnol. 31:230-232(2013) [PubMed] [Europe PMC]