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M.Smal is an N4-methylcytosine specific DNA-methylase.

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Klimasauskas S., Steponaviciene D., Maneliene Z., Petrusyte M., Butkus V., Janulaitis A.

An enzymatic activity rendering DNA immune to the action of the Smal restriction endonuclease in the presence of S-adenosyl-L-methionine has been detected in Serratia marcescens Sb. This methylase, M.Smal, modifies the second cytosine residue of the substrate sequence CCCGGG yielding N4-methylcytosine.

Nucleic Acids Res. 18:6607-6609(1990) [ PubMed | SRS | CiteXplore ]