Nucleotide sequence of trpE, anthranilate synthase I gene, of Bacillus caldotenax.
trpE gene of Bacillus caldotenax was cloned by complementation of an Escherichia coli tryptophan auxotroph. The trpE encoding a 508 amino acid polypeptide is followed by trpD. S1-nuclease mapping of a trpE in vivo transcript suggested that a promoter preceding the trpE was utilized by E. coli. The deduced amino acid sequence of anthranilate synthase I (ASI) was compared with that of five other bacterials ASIs.
Biochim. Biophys. Acta 1090:348-350(1991) [ PubMed | SRS | CiteXplore ]
