Overexpression of cardiac actin with baculovirus is promoter dependent.
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Yates S.P., Otley M.D., Dawson J.F.
The influence of the promoter and an N-terminal hexahistidine tag on human cardiac actin (ACTC) expression and function was investigated using four baculovirus constructs. It was found that both non-tagged ACTC and hisACTC expression from the p10 promoter was higher than from the polh promoter. Characterization showed that an N-terminal hexahistidine tag has a negative effect on ACTC. Recombinant ACTC inhibits DNase-I and binds myosin S1, indicative of proper folding. Our data support the hypothesis that the actin protein down-regulates the polh promoter.
Arch. Biochem. Biophys. 466:58-65(2007) [PubMed] [Europe PMC]