Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.

Nam Y., Sliz P., Song L., Aster J.C., Blacklow S.C.

Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes.

Cell 124:973-983(2006) [PubMed] [Europe PMC]