Molecular cloning of a cDNA encoding a putative cuticle collagen of Trichinella spiralis.
Fu B.Q., Liu M.Y., Kapel C.M., Meng X.P., Lu Q., Wu X.P., Chen Q.J., Boireau P.
A 5-day-old adult stage-specific cDNA fragment from Trichinella spiralis was identified by suppression subtractive hybridization and was used as a probe to screen the cDNA library. The cDNA sequence coding for a putative T. spiralis cuticle collagen was isolated. The cDNA encoded an open reading frame of 343 amino acid residues with molecular weight of 35.1 k Da. The deduced protein contained an N-terminal signal peptide, a nematode cuticle collagen N-terminal domain and a collagen triple helix repeat domain. Searches in GenBank using BLASTP showed up to 47% identity to cuticle collagens from other nematodes. Southern blot analysis of genomic DNA indicated this gene was present as a single copy in T. spiralis genome.
Vet. Parasitol. 132:31-35(2005) [ PubMed | SRS | CiteXplore ]
