An acetate-sensitive mutant of Neurospora crassa deficient in acetyl-CoA hydrolase.
Connerton I.F., McCullough W., Fincham J.R.S.
The predicted amino acid sequence of the product of the acetate-inducible acu-8 gene of Neurospora crassa, previously of unknown function, has close homology to the recently published sequence of Saccharomyces cerevisiae acetyl-CoA hydrolase. An acu-8 mutant strain, previously characterized as acetate non-utilizing, shows strong growth-inhibition by acetate, but will use it as carbon source at low concentrations. The mutant was shown to be deficient in acetyl-CoA hydrolase and to accumulate acetyl-CoA when supplied with acetate. As in Saccharomyces, the Neurospora enzyme is acetate-inducible.
J. Gen. Microbiol. 138:1797-1800(1992) [ PubMed | SRS | CiteXplore ]
