Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3.
Martin-Gallardo A., McCombie W.R., Gocayne J.D., Fitzgerald M.G., Wallace S., Lee B.M., Lamerdin J.E., Trapp S., Kelley J.M., Liu L.-I., Dubnick M., Johnston-Dow L.A., Kerlavage A.R., de Jong P., Carrano A., Fields C., Venter J.C., et al.
A total of 116,118 basepairs (bp) derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods. The assembled sequence forms two contigs totalling 105,831 bp, which contain a human fosB proto-oncogene, a gene encoding a protein phosphatase, two genes of unknown function and the previously-characterized ERCC1 DNA repair gene. This light band region has a high average density of 1.4 Alu repeats per kilobase. Human chromosome light bands could therefore contain up to 75,000 genes and 1.5 million Alu repeats.
Nat. Genet. 1:34-39(1992) [ PubMed | SRS | CiteXplore ]
